The Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai, China.
Anim Biotechnol. 2010 Oct;21(4):217-25. doi: 10.1080/10495398.2010.505843.
Grass carp, Ctenopharyngodon idellus (Valenciennes, 1844), is an economically important species widely cultured in the world, but its genome research resources are largely lacking. The objectives of this study were to construct normalized cDNA libraries for efficient EST analysis, to generate ESTs from these libraries, and to identify EST-related molecular markers such as microsatellites and single nucleotide polymorphisms (SNPs) for genetic analysis of this species. A total of 6,269 ESTs were generated representing 4,815 unique sequences, from which 105 putative microsatellites and 5,228 SNPs were identified. These genome resources provide the material basis for future genetic and functional analyses in this species.
草鱼，鲤形目鲤科草鱼属（瓦伦西恩，1844 年），是一种经济价值很高的鱼类，广泛养殖于世界各地，但它的基因组研究资源却非常匮乏。本研究的目的是构建均一化 cDNA 文库以进行有效的 EST 分析，从这些文库中产生 EST，并鉴定 EST 相关的分子标记，如微卫星和单核苷酸多态性（SNP），用于该物种的遗传分析。共产生了 6269 条 EST，代表了 4815 个独特的序列，从中鉴定出 105 个可能的微卫星和 5228 个 SNP。这些基因组资源为该物种的未来遗传和功能分析提供了物质基础。
