College of Fisheries, Zhejiang Ocean University, Zhoushan 316022, China.
Zhejiang Marine Fisheries Research Institute, Zhoushan 316021, China.
Int J Mol Sci. 2023 Jul 19;24(14):11622. doi: 10.3390/ijms241411622.
In order to explore the molecular regulatory mechanism of temperature acclimation under long-term temperature stress in , this study used high-throughput sequencing technology to analyze 60 days of breeding under five temperature conditions (12 °C, 16 °C, 20 °C, 24 °C, 28 °C). Compared with 20 °C, 9202, 4959 differentially expressed genes (DEGs) were discovered in low-temperature groups (12 °C, 16 °C), whereas 133 and 878 DEGs were discovered in high-temperature groups (24 °C, 28 °C), respectively. The KEGG functional enrichment analysis revealed that DEGs were primarily enriched in tight junction, PI3 K-Akt signaling pathway and protein digestion and absorption in low-temperature groups, and mainly enriched in proximal tubule bicarbonate reclamation, protein digestion and absorption, and HIF-1 signaling pathway in high-temperature groups. The viability of transcriptome sequencing-based screening of DEGs for temperature adaptation in was shown by the selection of eight DEGs for further validation by quantitative real-time PCR (qRT-PCR), the findings of which were consistent with the RNA-seq data. According to the findings, protein digestion and absorption were primarily regulated by temperature variations, physiological stress was a significant regulator in regulation under high-temperature stress, and the immune system was a significant regulator in regulation under low-temperature stress. The transcriptional patterns of under temperature stress are revealed in this study. This knowledge is crucial for understanding how adapts to temperature and can help us better comprehend the environmental difficulties that adaptation faces.
为了探索长期温度胁迫下温度驯化的分子调控机制，本研究使用高通量测序技术分析了在五种温度条件（12°C、16°C、20°C、24°C、28°C）下 60 天的繁殖情况。与 20°C 相比，在低温组（12°C、16°C）中发现了 9202 个和 4959 个差异表达基因（DEGs），而在高温组（24°C、28°C）中分别发现了 133 个和 878 个 DEGs。KEGG 功能富集分析表明，DEGs 主要富集在紧密连接、PI3K-Akt 信号通路和蛋白质消化吸收中，而在高温组中主要富集在近端肾小管碳酸氢盐回收、蛋白质消化吸收和 HIF-1 信号通路中。通过定量实时 PCR（qRT-PCR）对 8 个 DEGs 进行进一步验证，证明了转录组测序筛选 DEGs 适应温度的可行性，结果与 RNA-seq 数据一致。研究结果表明，蛋白质消化吸收主要受温度变化调节，生理应激是高温胁迫下调节的重要调节剂，免疫系统是低温胁迫下调节的重要调节剂。本研究揭示了 在温度胁迫下的转录模式。这些知识对于理解 如何适应温度以及帮助我们更好地理解 适应环境的困难至关重要。
