Department of Laser Medicine, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
Exp Biol Med (Maywood). 2010 Feb;235(2):175-80. doi: 10.1258/ebm.2009.009294.
In this study, a fluorescence method was established to obtain the local concentration of a photosensitizer (PS) based on the realtime fluorescence measurement of skin with port wine stain (PWS) during photodynamic therapy (PDT). This algorithm corrected for the distortions of PS fluorescence spectra imposed by the absorption of melanin and hemoglobin in skin and other factors, which yields a semi-quantitative measurement of PS concentration. Based on this information, a therapeutic effect correlation index (TECI) was proposed as the area under the PS concentration-time curve during PDT. The correlation between TECI and PDT treatment outcome was analyzed from 31 PWS patients. The measured PS fluorescence spectra showed that under the same PS dose, there were clear variations in the concentrations of the PS during PDT. Statistical analysis showed that TECI has a positive correlation with PDT outcome. Patients with a higher TECI value had a better treatment outcome. These results suggest that fluorescence spectroscopy can be used in situ to monitor skin PS concentration during PDT and to provide a valuable diagnostic tool to predict PDT outcome.
在这项研究中，建立了一种荧光方法，通过对酒红色斑（PWS）皮肤在光动力治疗（PDT）过程中的实时荧光测量，获得光动力剂（PS）的局部浓度。该算法校正了 PS 荧光光谱因皮肤中黑色素和血红蛋白的吸收以及其他因素引起的扭曲，从而对 PS 浓度进行半定量测量。基于此信息，提出了治疗效果相关指数（TECI）作为 PDT 过程中 PS 浓度-时间曲线下的面积。从 31 名 PWS 患者中分析了 TECI 与 PDT 治疗结果之间的相关性。测量的 PS 荧光光谱表明，在相同的 PS 剂量下， PDT 过程中 PS 的浓度存在明显变化。统计分析表明，TECI 与 PDT 结果呈正相关。TECI 值较高的患者治疗效果更好。这些结果表明，荧光光谱法可用于实时监测 PDT 过程中皮肤 PS 浓度，并为预测 PDT 结果提供有价值的诊断工具。
