β-胡萝卜素类物质不能显著激活视黄酸受体α或β。
{beta}-Apocarotenoids do not significantly activate retinoic acid receptors {alpha} or {beta}.
机构信息
Department of Human Nutrition, The Ohio State University, Columbus, 43210, USA.
出版信息
Exp Biol Med (Maywood). 2010 Mar;235(3):342-8. doi: 10.1258/ebm.2009.009202.
beta-Carotene oxygenase 2 cleaves beta-carotene asymmetrically at non-central double bonds of the polyene chain, yielding apocarotenal molecules. The hypothesis tested was that apocarotenoids are able to stimulate transcription by activating retinoic acid receptors (RARs). The effects of long- and short-chain apocarotenals and apocarotenoic acids on the activation of RARalpha and RARbeta transfected into monkey kidney fibroblast cells (CV-1) were investigated. We synthesized or purified beta-apo-8'-carotenoic acid (apo-8'-CA), beta-apo-14'-carotenoic acid (apo-14'-CA), beta-cyclocitral (BCL), beta-cyclogernanic acid (BCA), beta-ionone (BI), beta-ionylideneacetaldehyde (BIA) beta-ionylideneacetic acid (BIAA) and a C13 ketone, beta-apo-13-carotenone (C13). None of the apocarotenoids tested showed significant transactivation activity for the RARs when compared with all-trans retinoic acid (RA). The results suggest that biological effects of these apocarotenoids are through mechanisms other than activation of RARalpha and beta.
β-胡萝卜素 2 加氧酶在多烯链的非中央双键处不对称地切割β-胡萝卜素，生成类胡萝卜素醛分子。本研究旨在验证类胡萝卜素是否能够通过激活视黄酸受体（RARs）来刺激转录。我们研究了长链和短链类胡萝卜素醛和类胡萝卜素酸对转染猴肾成纤维细胞（CV-1）的 RARalpha 和 RARbeta 的激活作用。我们合成或纯化了β-脱-8'-胡萝卜素酸（apo-8'-CA）、β-脱-14'-胡萝卜素酸（apo-14'-CA）、β-环柠檬醛（BCL）、β-环没药酸（BCA）、β-紫罗兰酮（BI）、β-紫罗兰酮亚基乙醛（BIA）、β-紫罗兰酮亚基乙酸（BIAA）和一种 C13 酮，β-脱-13-胡萝卜素酮（C13）。与全反式视黄酸（RA）相比，测试的类胡萝卜素均未显示出对 RARs 的显著转录激活活性。结果表明，这些类胡萝卜素的生物学效应不是通过激活 RARalpha 和 RARbeta 来实现的。
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