Liu Jia-Ren, Chen Bing-Qing, Yang Bao-Feng, Dong Hong-Wei, Sun Chang-Hao, Wang Qi, Song Guo, Song You-Qiang
Public Health College, Harbin Medical University, Harbin 15001, Heilongjiang Province, China.
World J Gastroenterol. 2004 Feb 1;10(3):348-51. doi: 10.3748/wjg.v10.i3.348.
To investigate the effect of beta-ionone on the growth and apoptosis of gastric adenocarcinoma cell line SGC-7901.
Using MTT, fluorescence dye (Hoechst-33258), transmission electron microscopy and the TUNEL assay, we examined growth and apoptosis of SGC-7901 cells treated with beta-ionone at various concentrations (i.e. 25, 50, 100 and 200 micromol/L) for 24 h, 48 h.
The growth of SGC-7901 cells was inhibited by beta-ionone. Seven days after treatment with beta-ionone at four concentrations, the inhibition rates were 12.04%, 30.59%, 78.25% and 94.15%, respectively. The IC(50) value of beta-ionone for SGC-7901 cells was estimated to be 89 micromol/L. The apoptotic morphology was demonstrated in SGC-7901 cells treated with beta-ionone by Hoechst-33258 staining and electron microscopy. Apoptosis was also shown in beta-ionone-treated SGC-7901 cells by the TUNEL assay.
beta-ionone can inhibit cell proliferation and induce apoptosis of SGC-7901 cells. However, the mechanism needs to be further investigated.
研究β-紫罗兰酮对胃腺癌细胞系SGC-7901生长和凋亡的影响。
采用MTT法、荧光染料（Hoechst-33258）、透射电子显微镜及TUNEL法，检测不同浓度（25、50、100和200 μmol/L）的β-紫罗兰酮处理SGC-7901细胞24小时、48小时后的生长和凋亡情况。
β-紫罗兰酮可抑制SGC-7901细胞的生长。用四种浓度的β-紫罗兰酮处理细胞7天后，抑制率分别为12.04%、30.59%、78.25%和94.15%。β-紫罗兰酮对SGC-7901细胞的IC(50)值估计为89 μmol/L。通过Hoechst-33258染色和电子显微镜观察发现，经β-紫罗兰酮处理的SGC-7901细胞呈现凋亡形态。TUNEL法也显示β-紫罗兰酮处理的SGC-7901细胞发生了凋亡。
β-紫罗兰酮可抑制SGC-7901细胞的增殖并诱导其凋亡。然而，其机制有待进一步研究。
