通过等位基因置换分离得到的金黄色葡萄球菌中毒性休克综合征毒素突变体在兔子宫模型中缺乏毒力。
A toxic shock syndrome toxin mutant of Staphylococcus aureus isolated by allelic replacement lacks virulence in a rabbit uterine model.
作者信息
Sloane R, de Azavedo J C, Arbuthnott J P, Hartigan P J, Kreiswirth B, Novick R, Foster T J
机构信息
Microbiology Department, Moyne Institute, Trinity College, Dublin, Ireland.
出版信息
FEMS Microbiol Lett. 1991 Mar 1;62(2-3):239-44. doi: 10.1016/0378-1097(91)90164-6.
The gene coding for toxic shock syndrome toxin-1 in S. aureus was inactivated by allelic replacement in two TSS-associated strains. One mutant derived from FRI1169 (a non-enterotoxigenic strain) lacked virulence in the rabbit uterine chamber infection model. This suggests that TSST-1 is the only determinant produced by this strain that can induce the symptoms of shock in rabbits. A novel method for allelic replacement involving transduction of plasmid integrants is described.
在两株与中毒性休克综合征（TSS）相关的金黄色葡萄球菌菌株中，通过等位基因替换使编码中毒性休克综合征毒素-1的基因失活。一株源自FRI1169（非产肠毒素菌株）的突变体在兔子宫腔感染模型中缺乏毒力。这表明TSST-1是该菌株产生的唯一可诱发兔休克症状的决定因素。本文描述了一种涉及转导质粒整合体的等位基因替换新方法。
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