利用兔子宫模型在体内克隆毒性休克综合征毒素1基因（tst）的表达
Expression of the cloned toxic shock syndrome toxin 1 gene (tst) in vivo with a rabbit uterine model.
作者信息
de Azavedo J C, Foster T J, Hartigan P J, Arbuthnott J P, O'Reilly M, Kreiswirth B N, Novick R P
出版信息
Infect Immun. 1985 Oct;50(1):304-9. doi: 10.1128/iai.50.1.304-309.1985.
Abstract
Toxic shock syndrome (TSS) toxin 1 (TSST1) is produced by strains of Staphylococcus aureus associated with TSS. Purified TSST1 induces in rabbits a shock-like illness with many features similar to TSS in humans. These symptoms were also induced by TSST1-producing bacteria in diffusion chambers implanted in the rabbit uterus. Naturally occurring TSST1+ strains and a TSST1- strain harboring a pE194-derived plasmid carrying the cloned TSST1 determinant tst gave the same symptoms. TSST1- strains and a TSST1- strain carrying a pE194-tst plasmid with a deletion of the tst gene had no effect in rabbits. The results with the plasmid-carrying TSST1+ and TSST1- strains, which were isogenic apart from tst, show that the toxin is responsible for the illness in rabbits and suggest that it is a major factor in the pathogenesis of TSS.
摘要
中毒性休克综合征（TSS）毒素1（TSST1）由与TSS相关的金黄色葡萄球菌菌株产生。纯化的TSST1可在兔子身上诱发类似休克的疾病，其许多特征与人类的TSS相似。植入兔子子宫的扩散小室中的产TSST1细菌也会诱发这些症状。自然产生的TSST1+菌株以及携带克隆的TSST1决定簇tst的pE194衍生质粒的TSST1-菌株产生相同的症状。TSST1-菌株以及携带缺失tst基因的pE194-tst质粒的TSST1-菌株对兔子没有影响。携带TSST1+和TSST1-质粒的菌株（除tst外基因相同）的结果表明，该毒素是兔子发病的原因，并提示它是TSS发病机制中的主要因素。
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