Sobczak Agnieszka, Kowalski Zygmunt, Wzorek Zbigniew
Institute of Inorganic Chemistry and Technology, Cracow University of Technology, Cracow, Poland.
Acta Bioeng Biomech. 2009;11(4):23-8.
This paper presents the method of obtaining hydroxyapatite from animal bones. Bone sludge and calcined products were characterized by X-ray diffraction (XRD), Fourier transformed infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). Calcium concentration was determined with titration, and phosphorus--spectrophotometrically. Making use of the AAS and ICP methods the content of microelements was determined. In all the products, hydroxyapatite was the only crystalline phase indicated. The FT-IR spectra confirmed that calcination removed the total of organic substances. Calcium and phosphorus contents were 38% and 18%, respectively, which corresponded to the Ca/P molar ratio of nonstoichiometric hydroxyapatite. The specific surfaces of products were measured by BET method. The volume of micro- and mesopores was determined.
本文介绍了从动物骨骼中获取羟基磷灰石的方法。通过X射线衍射(XRD)、傅里叶变换红外光谱(FT-IR)和扫描电子显微镜(SEM)对骨泥和煅烧产物进行了表征。通过滴定法测定钙浓度,用分光光度法测定磷浓度。利用原子吸收光谱法(AAS)和电感耦合等离子体法(ICP)测定微量元素含量。在所有产物中,羟基磷灰石是唯一显示出的晶相。FT-IR光谱证实煅烧去除了所有有机物质。钙和磷的含量分别为38%和18%,这与非化学计量羟基磷灰石的Ca/P摩尔比相对应。通过BET法测量产物的比表面积。测定了微孔和介孔的体积。