Department of Chemistry, Pukyong National University, Busan 608-737, Korea.
Biomed Mater. 2011 Jun;6(3):035003. doi: 10.1088/1748-6041/6/3/035003. Epub 2011 Apr 13.
In the present study, hydroxyapatite (HAp) was isolated from Thunnus obesus bone using alkaline hydrolysis and thermal calcination methods. The obtained ceramic has been characterized by thermal gravimetric analysis (TGA), Fourier transform infrared spectroscopy (FT-IR), powder x-ray diffraction analysis (XRD), field-emission scanning electron microscopy, energy-dispersive x-ray analysis, transmission electron microscopy (TEM), selected area diffraction analysis, cytotoxic analysis and cell proliferation analysis. The results indicate that there are significant differences between the ceramics and T. obesus bone. FT-IR and TGA results affirmed that the collagen and organic moieties have been eliminated by both the proposed methods. XRD results were in agreement with JCPDS data. TEM and selective area diffraction images have signified that the thermal calcination method produces good crystallinity with dimensions 0.3-1.0 µm, whereas the alkaline hydrolysis method produces nanostructured HAp crystals with 17-71 nm length and 5-10 nm width. Biocompatibility of HAp crystals was evaluated by cytotoxicity and cell proliferation with human osteoblast-like cell MG-63.
在本研究中，使用碱性水解和热煅烧方法从鲐鱼骨骼中分离出羟基磷灰石（HAp）。通过热重分析（TGA）、傅里叶变换红外光谱（FT-IR）、粉末 X 射线衍射分析（XRD）、场发射扫描电子显微镜、能谱分析、透射电子显微镜（TEM）、选区衍射分析、细胞毒性分析和细胞增殖分析对获得的陶瓷进行了表征。结果表明，陶瓷与鲐鱼骨骼之间存在显著差异。FT-IR 和 TGA 结果证实，这两种方法都去除了胶原蛋白和有机部分。XRD 结果与 JCPDS 数据一致。TEM 和选区衍射图像表明，热煅烧法可产生具有 0.3-1.0 µm 尺寸的良好结晶度，而碱性水解法可产生具有 17-71 nm 长度和 5-10 nm 宽度的纳米结构 HAp 晶体。通过人成骨样细胞 MG-63 进行细胞毒性和细胞增殖评估 HAp 晶体的生物相容性。
