组织型纤溶酶原激活物对实验性缺血性脑卒中的基因反应。
Genome response to tissue plasminogen activator in experimental ischemic stroke.
机构信息
Department of Neurology and M,I,N,D, Institute, University of California at Davis, Sacramento, California 95817, USA.
出版信息
BMC Genomics. 2010 Apr 21;11:254. doi: 10.1186/1471-2164-11-254.
BACKGROUND
Tissue plasminogen activator (tPA) is known to have functions beyond fibrinolysis in acute ischemic stroke, such as blood brain barrier disruption. To further delineate tPA functions in the blood, we examined the gene expression profiles induced by tPA in a rat model of ischemic stroke.
RESULTS
tPA differentially expressed 929 genes in the blood of rats (p <or= 0.05, fold change >or= |1.2|). Genes identified had functions related to modulation of immune cells. tPA gene expression was found to be dependent on the reperfusion status of cerebral vasculature. The majority of genes regulated by tPA were different from genes regulated by ischemic stroke.
CONCLUSIONS
tPA modulates gene expression in the blood of rats involving immune cells in a manner that is dependent on the status of vascular reperfusion. These non-fibrinolytic activities of tPA in the blood serve to better understand tPA-related complications.
背景
组织型纤溶酶原激活物（tPA）除了具有纤溶作用外，在急性缺血性脑卒中中有多种功能，如破坏血脑屏障。为了进一步阐明 tPA 在血液中的作用，我们在缺血性脑卒中大鼠模型中检测了 tPA 诱导的基因表达谱。
结果
tPA 在大鼠血液中差异表达了 929 个基因（p<0.05，倍数变化>或=|1.2|）。鉴定出的基因具有与免疫细胞调节相关的功能。tPA 基因表达依赖于脑血管再灌注状态。tPA 调节的大多数基因与缺血性脑卒中调节的基因不同。
结论
tPA 以依赖于血管再灌注状态的方式调节大鼠血液中的基因表达，涉及免疫细胞。tPA 在血液中的这些非纤溶活性有助于更好地理解与 tPA 相关的并发症。
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