弗瑞德红白血病细胞与小鼠肝癌细胞之间的体细胞杂种。

Somatic cell hybrids between Friend erythroleukemia cells and mouse hepatoma cells.

作者信息

Conscience J F, Ruddle F H, Skoultchi A, Darlington G J

出版信息

Somatic Cell Genet. 1977 Mar;3(2):157-72. doi: 10.1007/BF01551812.

PMID:204066

Abstract

Somatic cell hybrids between hepatoma and Friend erythroleukemia parental cells were studied for the expression of liver-specific and erythroid properties. Several independent clones were isolated using HAT selection and were shown to be true hybrids by isozyme and chromosome analysis. All displayed a complete extinction of hemoglobin and globin mRNA production, but a retention of albumin and transferrin secretion. The data suggest that erythroid differntiation is being actively inhibited by the hepatoma genome. Possible mechanisms that might explain these results are discussed in the light of current hypotheses regarding the mechanism of cell differentiation.

摘要

对肝癌和亲代Friend红白血病细胞之间的体细胞杂种进行了研究，以观察肝脏特异性和红系特性的表达。使用HAT选择分离出几个独立的克隆，并通过同工酶和染色体分析表明它们是真正的杂种。所有克隆均显示血红蛋白和珠蛋白mRNA产生完全消失，但白蛋白和转铁蛋白分泌得以保留。数据表明红系分化受到肝癌基因组的积极抑制。根据当前关于细胞分化机制的假说，讨论了可能解释这些结果的机制。