McBurney M W, Featherstone M S, Kaplan H
Cell. 1978 Dec;15(4):1323-30. doi: 10.1016/0092-8674(78)90057-0.
Hybrid cells formed by the fusion of murine teratocarcinoma and Friend erythroleukemia cells synthesize hemoglobin in the presence of chemical inducers such as dimethylsulfoxide (DMSO). By making use of the fact that the parental teratocarcinoma and Friend cells carried different alleles at the locus coding for the alpha chain of hemoglobin, it was possible to demonstrate that the teratocarcinoma-derived genes for the globin alpha chains are genetically active in hemoglobin-synthesizing hybrid cells. In addition, evidence is presented suggesting that the teratocarcinoma-derived genes for the beta-globin chains may also be expressed in the hybrids. Apparently the teratocarcinoma-derived genome has become reprogrammed to express erythroid functions following fusion of the teratocarcinoma cell to the Friend cell.
由小鼠畸胎瘤细胞和弗氏红白血病细胞融合形成的杂交细胞，在化学诱导剂如二甲基亚砜（DMSO）存在的情况下合成血红蛋白。利用亲代畸胎瘤细胞和弗氏细胞在编码血红蛋白α链的基因座上携带不同等位基因这一事实，有可能证明来自畸胎瘤的珠蛋白α链基因在合成血红蛋白的杂交细胞中具有遗传活性。此外，有证据表明来自畸胎瘤的β-珠蛋白链基因在杂交细胞中也可能表达。显然，在畸胎瘤细胞与弗氏细胞融合后，来自畸胎瘤的基因组已被重新编程以表达红系功能。
