Peterson J A, Weiss M C
Proc Natl Acad Sci U S A. 1972 Mar;69(3):571-5. doi: 10.1073/pnas.69.3.571.
The synthesis of serum albumin has been studied in hybrids between well-differentiated rat hepatoma cells, which synthesize serum albumin, and mouse fibroblasts (3T3) that do not synthesize albumin. By immunodiffusion techniques with noncrossreacting antisera, the production of both rat and mouse albumin by the hybrids has been examined. Karyologically identified hybrids were produced between 3T3 cells and cells of a 1s hepatoma (Fu5) clone, and of a 2s hepatoma (2s Fu5-5cl.lE) clone. Each of the 3T3 x Fu5 hybrids produces only rat albumin. Among five 3T3 x 2s Fu5-5cl.lE hybrid clones isolated, one produces both rat and mouse albumin, two produce only mouse albumin, and two do not produce rat or mouse albumin.
已对高分化大鼠肝癌细胞（能合成血清白蛋白）与不合成白蛋白的小鼠成纤维细胞（3T3）之间的杂交细胞中血清白蛋白的合成进行了研究。通过使用非交叉反应抗血清的免疫扩散技术，检测了杂交细胞中大鼠和小鼠白蛋白的产生情况。通过染色体分析鉴定出的杂交细胞是在3T3细胞与1s肝癌（Fu5）克隆细胞以及2s肝癌（2s Fu5 - 5cl.lE）克隆细胞之间产生的。每个3T3×Fu5杂交细胞只产生大鼠白蛋白。在分离出的五个3T3×2s Fu5 - 5cl.lE杂交细胞克隆中，一个同时产生大鼠和小鼠白蛋白，两个只产生小鼠白蛋白，另外两个既不产生大鼠白蛋白也不产生小鼠白蛋白。
