肝癌-成纤维细胞杂交瘤中血清蛋白分泌的消退、保留和诱导

Extinction, retention and induction of serum protein secretion in hepatoma-fibroblast hybrids.

作者信息

Szpirer C, Szpirer J

出版信息

Differentiation. 1976 Jun 4;5(2-3):97-9. doi: 10.1111/j.1432-0436.1976.tb00898.x.

DOI:10.1111/j.1432-0436.1976.tb00898.x

Abstract

The production of four serum proteins has been analysed in several hepatoma-fibroblast hybrids. Extinction of albumin and alpha-foetoprotein production occurs systematically in intra and interspecific (rat X mouse) hybrids derived from mouse hepatoma cells (BW). Similar hybrids derived from two related clones of rat hepatoma cells either do not produce albumin (Fa32-derived hybrids), as the BW-derived hybrids, or retain the capacity to produce it, but at a reduced rate (Fu5-derived hybrids); some differences in the control of albumin production thus seem to exist between clonal hepatoma cell lines. The mouse hepatoma cell hybrids retain the capacity to secrete transferrin at a reduced rate, and C3 (the third component of complement) at a high rate. Further analysis of C3 production in interspecific hybrids showed that both parental genomes actively contribute to C3 production: induction of C3 secretion is thus observed in these hybrids.

摘要

在几种肝癌-成纤维细胞杂种中分析了四种血清蛋白的产生情况。在源自小鼠肝癌细胞（BW）的种内和种间（大鼠×小鼠）杂种中，白蛋白和甲胎蛋白的产生系统性地消失。源自大鼠肝癌细胞两个相关克隆的类似杂种，要么像源自BW的杂种一样不产生白蛋白（源自Fa32的杂种），要么保留产生白蛋白的能力，但速率降低（源自Fu5的杂种）；因此，克隆肝癌细胞系之间在白蛋白产生的调控上似乎存在一些差异。小鼠肝癌细胞杂种保留了以较低速率分泌转铁蛋白和以高速率分泌C3（补体第三成分）的能力。对种间杂种中C3产生的进一步分析表明，两个亲本基因组都对C3的产生有积极贡献：因此在这些杂种中观察到了C3分泌的诱导。