Shioya H, Shimojo M, Kawahara Y
Product Development Laboratories, Sankyo Co., Ltd., Tokyo, Japan.
J Immunoassay. 1991;12(1):15-28. doi: 10.1080/01971529108055054.
A simple and sensitive enzyme immunoassay (EIA) for determination of the active metabolite (RS-5139) of a new angiotensin converting enzyme inhibitor (CS-622) was developed. The N-succinimmidyl ester of RS-5139 was coupled with bovine serum albumin (BSA) and its conjugate was used as an immunogen. Horse radish peroxidase (HRP; EC 1, 11, 1, 7) was used as a labeled enzyme and 3, 3', 5, 5'-tetramethylbenzidine was used as a substrate. The antiserum was used at a final dilution of 1:10000 and sensitivity was 10 pg in plasma and 20pg in urine. CS-622 exhibited cross-reactivity (22.3%), but other ACE inhibitors didn't exhibit cross-reactivity. The plasma levels determined by EIA and GC/MS was good agreement (y = 6.58e-2 + 1.07x, R - 2 = 0.985, n = 52).
开发了一种简单灵敏的酶免疫分析法（EIA），用于测定新型血管紧张素转换酶抑制剂（CS-622）的活性代谢物（RS-5139）。将RS-5139的N-琥珀酰亚胺酯与牛血清白蛋白（BSA）偶联，其偶联物用作免疫原。辣根过氧化物酶（HRP；EC 1, 11, 1, 7）用作标记酶，3, 3', 5, 5'-四甲基联苯胺用作底物。抗血清的最终稀释度为1:10000，血浆中的灵敏度为10 pg，尿液中的灵敏度为20 pg。CS-622表现出交叉反应性（22.3%），但其他血管紧张素转换酶抑制剂未表现出交叉反应性。通过EIA和GC/MS测定的血浆水平具有良好的一致性（y = 6.58e-2 + 1.07x，R - 2 = 0.985，n = 52）。
