使用聚合酶链反应（PCR）和酶联免疫吸附测定（ELISA）评估2型糖尿病微血管并发症患者的游离DNA。
Assessment of cell-free DNA with microvascular complication of type II diabetes mellitus, using PCR and ELISA.
作者信息
El Tarhouny Shereen A, Hadhoud Khaled M, Ebrahem Magdy M, Al Azizi Nashwa M
机构信息
Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
出版信息
Nucleosides Nucleotides Nucleic Acids. 2010 Mar;29(3):228-36. doi: 10.1080/15257771003708298.
In order to assess the potential biochemical markers in the development, diagnosis, and prognosis of diabetic patient with microvascular complication represented with retinopathy, we analyzed the levels of cell-free DNA by two different techniques. The levels of cell-free GAPDH assayed by quantitative PCR were significantly higher in the plasma samples of diabetic patients with and without diabetic retinopathy than in those of the control group; thus, it is a better biomarker than nucleosomes assayed by ELISA in patients with type 2 diabetes for the early detection of development of microvascular complications as retinopathy.
为了评估以视网膜病变为代表的糖尿病微血管并发症患者在病情发展、诊断和预后方面的潜在生化标志物，我们采用两种不同技术分析了游离DNA水平。通过定量PCR检测的游离甘油醛-3-磷酸脱氢酶（GAPDH）水平在有或无糖尿病视网膜病变的糖尿病患者血浆样本中均显著高于对照组；因此，对于2型糖尿病患者微血管并发症如视网膜病变发展的早期检测，它是比通过酶联免疫吸附测定（ELISA）检测的核小体更好的生物标志物。
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