Department of Materials Science and Engineering, University of Florida, Gainesville, Florida 32611, USA.
Biointerphases. 2007 Jun;2(2):89-94. doi: 10.1116/1.2751405.
The surface of an indwelling medical device can be colonized by human pathogens that can form biofilms and cause infections. In most cases, these biofilms are resistant to antimicrobial therapy and eventually necessitate removal or replacement of the device. An engineered surface microtopography based on the skin of sharks, Sharklet AF, has been designed on a poly(dimethyl siloxane) elastomer (PDMSe) to disrupt the formation of bacterial biofilms without the use of bactericidal agents. The Sharklet AF PDMSe was tested against smooth PDMSe for biofilm formation of Staphylococcus aureus over the course of 21 days. The smooth surface exhibited early-stage biofilm colonies at 7 days and mature biofilms at 14 days, while the topographical surface did not show evidence of early biofilm colonization until day 21. At 14 days, the mean value of percent area coverage of S. aureus on the smooth surface was 54% compared to 7% for the Sharklet AF surface (p<0.01). These results suggest that surface modification of indwelling medical devices and exposed sterile surfaces with the Sharklet AF engineered topography may be an effective solution in disrupting biofilm formation of S. aureus.
留置医疗器械的表面可能会被人体病原体定植，这些病原体可以形成生物膜并导致感染。在大多数情况下，这些生物膜对抗菌治疗有抵抗力，最终需要移除或更换设备。一种基于鲨鱼皮肤的工程表面微形貌（Sharklet AF）已被设计在聚二甲基硅氧烷弹性体（PDMSe）上，以在不使用杀菌剂的情况下破坏细菌生物膜的形成。在 21 天的时间里，Sharklet AF PDMSe 被测试用于金黄色葡萄球菌在光滑 PDMSe 上的生物膜形成。光滑表面在 7 天显示出早期生物膜菌落，在 14 天显示出成熟生物膜，而形貌表面直到第 21 天才显示出早期生物膜定植的证据。在 14 天，光滑表面上金黄色葡萄球菌的面积覆盖率平均值为 54%，而 Sharklet AF 表面为 7%（p<0.01）。这些结果表明，用 Sharklet AF 工程形貌对留置医疗器械和暴露的无菌表面进行表面改性可能是破坏金黄色葡萄球菌生物膜形成的有效解决方案。
