Department of Chemistry, Texas A&M University, College Station, Texas 77843, USA.
Biointerphases. 2009 Dec;4(4):80-5. doi: 10.1116/1.3284738.
Herein, a simple label-free biosensor fabrication method is demonstrated based on transmission localized surface plasmon resonance (T-LSPR). The platform, which consists of a silver nanoparticle array, can be prepared in just a few minutes using benchtop chemistry. The array was made by a templating technique in conjunction with the photoreduction of Ag ions from solution. This metal surface was functionalized with biotin-linked thiol ligands for binding streptavidin molecules from solution. For an array of 19 nm diameter silver nanoparticles, a redshift in the T-LSPR spectrum of 24 nm was observed upon protein-ligand binding at saturation. The binding constant was found to be 2x10(12) M(-1). Platforms were also fabricated with silver nanoparticles of 34, 55, and 72 nm diameters. The maximum LSPR wavelength shift was nanoparticle size dependent and the maximum sensitivity was obtained with the smaller nanoparticles.
本文展示了一种基于传输局域表面等离子体共振（T-LSPR）的简单无标记生物传感器制造方法。该平台由银纳米粒子阵列组成，仅使用台式化学即可在短短几分钟内制备。该阵列通过模板技术与溶液中 Ag 离子的光还原相结合制成。该金属表面用生物素连接的硫醇配体官能化，用于从溶液中结合链霉亲和素分子。对于直径为 19nm 的银纳米粒子阵列，在蛋白质-配体结合达到饱和时，T-LSPR 光谱观察到 24nm 的红移。发现结合常数为 2x10(12) M(-1)。还使用 34nm、55nm 和 72nm 直径的银纳米粒子制造了平台。最大 LSPR 波长位移与纳米粒子尺寸有关，并且在较小的纳米粒子中获得了最大的灵敏度。
