Department of Chemistry, Lehigh University, 6 East Packer Avenue, Bethlehem, Pennsylvania 18015, United States.
Langmuir. 2024 Oct 1;40(39):20629-20639. doi: 10.1021/acs.langmuir.4c02543. Epub 2024 Sep 17.
Supported lipid bilayers (SLBs) are useful structures for mimicking cellular membranes, and they can be integrated with a variety of sensors. Although there are a variety of methods for forming SLBs, many of these methods come with limitations in terms of the lipid compositions that can be employed and the substrates upon which the SLBs can be deposited. Here we demonstrate the use of an all-aqueous chaotropic agent exchange process that can be used to form SLBs on two different substrate materials: SiO, which is compatible with traditional SLB formation by vesicle fusion, and AlO, which is not compatible with vesicle fusion. When examined with a quartz crystal microbalance with dissipation monitoring, the SLBs generated by chaotropic agent exchange (CASLBs) have similar frequency and dissipation shifts to SLBs formed by the vesicle fusion technique. The CASLBs block nonspecific protein adsorption on the substrate and can be used to sense protein-lipid interactions. Fluorescence microscopy was used to examine the CASLBs, and we observed long-range lateral diffusion of fluorescent probes, which confirmed that the CASLBs were composed of a continuous, planar lipid bilayer. Our CASLB method provides another option for forming planar lipid bilayers on a variety of surfaces, including those that are not amenable to the widely used vesicle fusion method.
支持的脂质双层 (SLB) 是模拟细胞膜的有用结构，它们可以与各种传感器集成。虽然有多种形成 SLB 的方法，但其中许多方法在可使用的脂质组成和可沉积 SLB 的基板方面存在局限性。在这里，我们展示了一种全水相的变构剂交换过程，该过程可用于在两种不同的基板材料上形成 SLB：SiO，它与通过囊泡融合形成传统 SLB 兼容，而 AlO 则与囊泡融合不兼容。当用石英晶体微天平监测耗散时，通过变构剂交换 (CASLB) 形成的 SLB 具有与通过囊泡融合技术形成的 SLB 相似的频率和耗散变化。CASLB 阻止基板上非特异性蛋白质的吸附，并可用于感测蛋白质-脂质相互作用。荧光显微镜用于检查 CASLB，我们观察到荧光探针的长程侧向扩散，这证实了 CASLB 由连续的平面脂质双层组成。我们的 CASLB 方法为在各种表面上形成平面脂质双层提供了另一种选择，包括那些不适用于广泛使用的囊泡融合方法的表面。
