Gomes Francisca L, Wasserberg Dorothee, Edelbroek Rick, van Weerd Jasper, Jonkheijm Pascal, Leijten Jeroen
Department of Bioengineering Technologies, Leijten Laboratory, Faculty of Science and Technology, Technical Medical Centre, University of Twente, Drienerlolaan 5, Enschede 7522NB, The Netherlands.
Department of Molecules and Materials, Laboratory of Biointerface Chemistry, Faculty of Science and Technology, Technical Medical Centre and MESA+ Institute, University of Twente, Drienerlolaan 5, Enschede 7522NB, The Netherlands.
ACS Appl Mater Interfaces. 2025 Mar 26;17(12):18179-18193. doi: 10.1021/acsami.5c02103. Epub 2025 Mar 13.
Particles are essential building blocks in nanomedicine and cell engineering. Their administration often involves blood contact, which demands a hemocompatible material profile. Coating particles with isolated cell membranes is a common strategy to improve hemocompatibility, but this solution is nonscalable and potentially immunogenic. Cell membrane-like lipid coatings are a promising alternative, as lipids can be synthesized on a large scale and used to create safe cell membrane-like supported bilayers. However, a method to controllably and scalably lipid-coat a wide range of particles has remained elusive. Here, an on-particle solvent-assisted lipid coating (OPSALC) method is introduced as an innovative technique to endow various types of particles with cell membrane-like coatings. Coating formation efficiency is shown to depend on lipid concentration, buffer addition rate, and solvent:buffer ratio, as these parameters determine lipid assembly and lipid-surface interactions. Four lipid formulations with various levels of erythrocyte membrane mimicry are explored in terms of hemocompatibility, demonstrating a reduced particle-induced hemolysis and plasma coagulation time. Interestingly, formulations with higher mimicry levels show the lowest levels of complement activation and highest colloidal stability. Overall, OPSALC represents a simple yet scalable strategy to endow particles with cell membrane-like lipid coatings to facilitate blood-contact applications.
颗粒是纳米医学和细胞工程中的基本构建单元。它们的给药通常涉及与血液接触，这就需要具有血液相容性的材料特性。用分离的细胞膜包覆颗粒是提高血液相容性的常用策略，但这种方法不可扩展且可能具有免疫原性。类似细胞膜的脂质涂层是一种很有前景的替代方案，因为脂质可以大规模合成并用于创建安全的类似细胞膜的支撑双层膜。然而，一种可控制且可扩展地对各种颗粒进行脂质包覆的方法仍然难以捉摸。在此，引入了一种颗粒上溶剂辅助脂质涂层（OPSALC）方法，作为一种创新技术，可为各种类型的颗粒赋予类似细胞膜的涂层。涂层形成效率显示取决于脂质浓度、缓冲液添加速率和溶剂与缓冲液的比例，因为这些参数决定了脂质组装和脂质与表面的相互作用。研究了四种具有不同程度红细胞膜模拟性的脂质配方的血液相容性，结果表明颗粒诱导的溶血和血浆凝固时间减少。有趣的是，模拟水平较高的配方显示出最低水平的补体激活和最高的胶体稳定性。总体而言，OPSALC代表了一种简单但可扩展的策略，可为颗粒赋予类似细胞膜的脂质涂层，以促进血液接触应用。
