Lavrič Marta, Bar Laure, Villanueva Martin E, Losada-Pérez Patricia, Iglič Aleš, Novak Nikola, Cordoyiannis George
Condensed Matter Physics Department, Jožef Stefan Institute, 1000 Ljubljana, Slovenia.
Laboratory of Physics, Faculty of Electrical Engineering, University of Ljubljana, 1000 Ljubljana, Slovenia.
Sensors (Basel). 2024 Sep 20;24(18):6093. doi: 10.3390/s24186093.
Supported lipid bilayers (SLBs) are low-complexity biomimetic membranes, serving as popular experimental platforms to study membrane organization and lipid transfer, membrane uptake of nanoparticles and biomolecules, and many other processes. Quartz crystal microbalance with dissipation monitoring has been utilized to probe the influence of several parameters on the quality of SLBs formed on Au- and SiO-coated sensors. The influence of the aqueous medium (i.e., buffer type) and the adsorption temperature, above and below the lipid melting point, is neatly explored for SLBs of 1,2-dimyristoyl-sn-glycero-3-phosphocholine and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine formed by a solvent exchange. Below the lipid melting temperature, quality variations are observed upon the formation on Au and SiO surfaces, with the SLBs being more homogeneous for the latter. We further investigate how the buffer affects the detection of lipid melting in SLBs, a transition that necessitates high-sensitivity and time-consuming surface-sensitive techniques to be detected.
支撑脂质双层（SLBs）是低复杂性的仿生膜，是研究膜组织和脂质转移、纳米颗粒和生物分子的膜摄取以及许多其他过程的常用实验平台。具有耗散监测功能的石英晶体微天平已被用于探究几个参数对在金和二氧化硅涂层传感器上形成的SLBs质量的影响。对于通过溶剂交换形成的1,2-二肉豆蔻酰-sn-甘油-3-磷酸胆碱和1,2-二棕榈酰-sn-甘油-3-磷酸胆碱的SLBs，在脂质熔点上下整齐地探究了水性介质（即缓冲液类型）和吸附温度的影响。在脂质熔化温度以下，在金和二氧化硅表面形成时观察到质量变化，对于后者，SLBs更均匀。我们进一步研究了缓冲液如何影响SLBs中脂质熔化的检测，这种转变需要高灵敏度和耗时的表面敏感技术才能检测到。
