Departments of Psychology, Pharmacology and Toxicology, Institute for Neuroscience, University of Texas at Austin, Austin, TX, USA.
Photochem Photobiol. 2010 May-Jun;86(3):673-80. doi: 10.1111/j.1751-1097.2010.00732.x. Epub 2010 Apr 16.
Low-level light therapy (LLLT) increases survival of cultured cells, improves behavioral recovery from neurodegeneration and speeds wound healing. These beneficial effects are thought to be mediated by upregulation of mitochondrial proteins, especially the respiratory enzyme cytochrome oxidase. However, the effects of in vivo LLLT on cytochrome oxidase in intact skeletal muscle have not been previously investigated. We used a sensitive method for enzyme histochemistry of cytochrome oxidase to examine the rat temporalis muscle 24 h after in vivo LLLT. The findings showed for the first time that in vivo LLLT induced a dose- and fiber type-dependent increase in cytochrome oxidase in muscle fibers. LLLT was particularly effective at enhancing the aerobic capacity of intermediate and red fibers. The findings suggest that LLLT may enhance the oxidative energy metabolic capacity of different types of muscle fibers, and that LLLT may be used to enhance the aerobic potential of skeletal muscle.
低水平光疗法（LLLT）可提高培养细胞的存活率，改善神经退行性疾病的行为恢复，并加速伤口愈合。这些有益作用被认为是通过上调线粒体蛋白，特别是呼吸酶细胞色素氧化酶来介导的。然而，体内 LLLT 对完整骨骼肌中线粒体氧化酶的影响以前尚未被研究过。我们使用细胞色素氧化酶酶组织化学的敏感方法，在体内 LLLT 后 24 小时检查大鼠颞肌。研究结果首次表明，体内 LLLT 可诱导肌肉纤维中线粒体氧化酶的剂量和纤维类型依赖性增加。LLLT 特别有效地增强了中间纤维和红纤维的有氧能力。研究结果表明，LLLT 可能增强不同类型的肌肉纤维的氧化能量代谢能力，并且 LLLT 可能用于增强骨骼肌的有氧潜能。
