Key Lab for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
J Fish Biol. 2011 Jan;78(1):322-31. doi: 10.1111/j.1095-8649.2010.02793.x. Epub 2010 Dec 3.
Sixty novel simple sequence repeat (SSR) markers were developed from expressed sequence tags (EST) of half-smooth tongue sole Cynoglossus semilaevis exploited in the laboratory. The number of alleles, observed and expected heterozygosity per locus ranged from two to 16, from 0·0833 to 1·0000 and from 0·0816 to 0·913, respectively. Of these SSRs, 20 had significant homology to known genes by BLASTx (basic local alignment search tool x) search. For cross-species amplification, there are 53 positive amplifications in Japanese flounder Paralichthys olivaceus with 12 polymorphic loci and 51 positive amplifications in Senegalese sole Solea senegalensis with 11 polymorphic loci. These new EST-SSR markers will be useful for genetic studies and genome mapping of C. semilaevis and its closely related fishes.
从实验室养殖的半滑舌鳎 Cynoglossus semilaevis 的表达序列标签 (EST) 中开发了 60 个新的简单序列重复 (SSR) 标记。每个位点的等位基因数、观测和预期杂合度分别从 2 到 16、从 0.0833 到 1.0000 和从 0.0816 到 0.913。这些 SSR 中，有 20 个通过 BLASTx（基本局部比对搜索工具 x）搜索与已知基因具有显著同源性。对于跨物种扩增，在日本牙鲆 Paralichthys olivaceus 中有 53 个阳性扩增，12 个多态性位点，在塞内加尔鳎 Solea senegalensis 中有 51 个阳性扩增，11 个多态性位点。这些新的 EST-SSR 标记将有助于对半滑舌鳎及其近缘鱼类的遗传研究和基因组作图。
