Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, Potsdam-Golm, Germany.
Plant J. 2010 Mar;61(6):1067-91. doi: 10.1111/j.1365-313X.2010.04142.x.
Historically speaking, Arabidopsis was not the plant of choice for investigating photosynthesis, with physiologists and biochemists favouring other species such as Chlorella, spinach and pea. However, its inherent advantages for forward genetics rapidly led to its adoption for photosynthesis research. In the last ten years, the availability of the Arabidopsis genome sequence - still the gold-standard for plant genomes - and the rapid expansion of genetic and genomic resources have further increased its importance. Research in Arabidopsis has not only provided comprehensive information about the enzymes and other proteins involved in photosynthesis, but has also allowed transcriptional responses, protein levels and compartmentation to be analysed at a global level for the first time. Emerging technical and theoretical advances offer another leap forward in our understanding of post-translational regulation and the control of metabolism. To illustrate the impact of Arabidopsis, we provide a historical review of research in primary photosynthetic metabolism, highlighting the role of Arabidopsis in elucidation of the pathway of photorespiration and the regulation of RubisCO, as well as elucidation of the pathways of starch turnover and studies of the significance of starch for plant growth.
从历史上看，拟南芥并不是研究光合作用的首选植物，生理学家和生物化学家更喜欢其他物种，如绿藻、菠菜和豌豆。然而，它在正向遗传学方面的固有优势使其迅速被用于光合作用研究。在过去的十年中，拟南芥基因组序列的可用性——仍然是植物基因组的黄金标准——以及遗传和基因组资源的快速扩展进一步增加了它的重要性。在拟南芥中的研究不仅提供了关于光合作用中涉及的酶和其他蛋白质的全面信息，而且还首次允许在全局水平上分析转录反应、蛋白质水平和区室化。新兴的技术和理论进展为我们理解翻译后调控和代谢控制提供了另一个飞跃。为了说明拟南芥的影响，我们提供了对初级光合作用代谢研究的历史回顾，重点介绍了拟南芥在阐明光呼吸途径和 Rubisco 调节以及淀粉周转途径以及淀粉对植物生长的意义研究中的作用。
