Departament de Biologia, Microbiologia, Universitat de les Illes Balears, Campus UIB, 07122 Palma de Mallorca, Spain.
BMC Microbiol. 2010 Apr 21;10:118. doi: 10.1186/1471-2180-10-118.
The genus Pseudomonas comprises more than 100 species of environmental, clinical, agricultural, and biotechnological interest. Although, the recommended method for discriminating bacterial species is DNA-DNA hybridisation, alternative techniques based on multigenic sequence analysis are becoming a common practice in bacterial species discrimination studies. Since there is not a general criterion for determining which genes are more useful for species resolution; the number of strains and genes analysed is increasing continuously. As a result, sequences of different genes are dispersed throughout several databases. This sequence information needs to be collected in a common database, in order to be useful for future identification-based projects.
The PseudoMLSA Database is a comprehensive database of multiple gene sequences from strains of Pseudomonas species. The core of the database is composed of selected gene sequences from all Pseudomonas type strains validly assigned to the genus through 2008. The database is aimed to be useful for MultiLocus Sequence Analysis (MLSA) procedures, for the identification and characterisation of any Pseudomonas bacterial isolate. The sequences are available for download via a direct connection to the National Center for Biotechnology Information (NCBI). Additionally, the database includes an online BLAST interface for flexible nucleotide queries and similarity searches with the user's datasets, and provides a user-friendly output for easily parsing, navigating, and analysing BLAST results.
The PseudoMLSA database amasses strains and sequence information of validly described Pseudomonas species, and allows free querying of the database via a user-friendly, web-based interface available at http://www.uib.es/microbiologiaBD/Welcome.html. The web-based platform enables easy retrieval at strain or gene sequence information level; including references to published peer-reviewed articles, and direct external links to more specialized strain information databases (StrainInfo) and GeneBank (NCBI). The PseudoMLSA is intended to provide helpful strain-sequence information for a better and more comprehensive discriminative multigenic sequence based analysis of this special group of bacteria, contributing to enhance our understanding of the evolution of Pseudomonas species.
假单胞菌属包含 100 多种具有环境、临床、农业和生物技术应用价值的物种。尽管 DNA-DNA 杂交是鉴别细菌种的推荐方法，但基于多基因序列分析的替代技术在细菌种鉴别研究中已成为一种常见的做法。由于没有确定哪些基因更有助于种分辨率的通用标准，因此分析的菌株和基因数量在不断增加。结果，不同基因的序列分散在多个数据库中。为了在未来的基于鉴定的项目中有用，这些序列信息需要收集在一个通用的数据库中。
PseudoMLSA 数据库是一个包含假单胞菌属菌株多种基因序列的综合数据库。该数据库的核心由 2008 年以前通过有效鉴定到属的所有假单胞菌模式菌株的选定基因序列组成。该数据库旨在为多基因序列分析（MLSA）程序、任何假单胞菌细菌分离物的鉴定和特征分析提供帮助。用户可通过直接连接到国家生物技术信息中心（NCBI）下载序列。此外，该数据库还包括一个在线 BLAST 接口，用于灵活的核苷酸查询和与用户数据集的相似性搜索，并为用户提供了一个易于解析、导航和分析 BLAST 结果的用户友好输出。
PseudoMLSA 数据库汇集了有效描述的假单胞菌属菌株和序列信息，并通过用户友好的基于网络的界面允许免费查询数据库，网址为 http://www.uib.es/microbiologiaBD/Welcome.html。基于网络的平台可方便地在菌株或基因序列信息级别检索，包括对已发表的同行评议文章的引用，并提供直接外部链接到更专业的菌株信息数据库（StrainInfo）和基因库（NCBI）。PseudoMLSA 旨在为更好、更全面的基于多基因序列的鉴别分析提供有价值的菌株序列信息，有助于提高我们对假单胞菌属物种进化的理解。
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