通过多位点序列分型（MLST）方法揭示的摩洛哥[具体研究对象未给出]的遗传多样性和种群结构

Genetic Diversity and Population Structure of in Morocco as Revealed by Multilocus Sequence Typing (MLST) Approach.

作者信息

El Mazini Sara, Barhoumi Mourad, Mhaidi Idris, Daoui Othmane, Kbaich Mouad Ait, El Kacem Sofia, El Idrissi Saik Imane, Riyad Myriam, Bekhti Khadija, Guizani Ikram, Lemrani Meryem

机构信息

Laboratory of Parasitology and Vector-Borne-Diseases, Institut Pasteur du Morocco, Casablanca 20360, Morocco.

Laboratory of Microbial Biotechnology and Bioactive Molecules, Faculty of Sciences and Technologies, Sidi Mohammed Ben Abdellah University, Fes 30000, Morocco.

出版信息

Pathogens. 2023 May 31;12(6):785. doi: 10.3390/pathogens12060785.

is endemic in Morocco, and it causes both visceral (VL) and cutaneous leishmaniasis (CL). In this study, the multilocus sequence typing (MLST) approach was used to investigate the phylogeny and population structure of strains isolated from CL and VL patients and the canine reservoir in different leishmaniasis endemic foci in Morocco. For this purpose, eight loci (, , , , , , and ) were amplified in 40 samples, out of which 31 were successfully sequenced. The genetic diversity analysis detected a high degree of intraspecific genetic variability among the studied strains. The phylogenetic and the haplotype analyses showed that most of the strains from the same geographical areas clustered together. The recombination among strains was revealed through a splits tree analysis and the number of recombination events. Moreover, the assessment of the gene flow between and through phylogenetic analysis and haplotype diversity in two endemic foci where the two species were sympatric showed no genetic exchange between the two species.

在摩洛哥流行，可导致内脏利什曼病（VL）和皮肤利什曼病（CL）。在本研究中，采用多位点序列分型（MLST）方法，对从摩洛哥不同利什曼病流行病灶的CL和VL患者以及犬类宿主中分离出的菌株的系统发育和种群结构进行了研究。为此，在40个样本中扩增了8个基因座（、、、、、、和），其中31个成功测序。遗传多样性分析检测到所研究菌株之间存在高度的种内遗传变异性。系统发育和单倍型分析表明，来自相同地理区域的大多数菌株聚集在一起。通过分裂树分析和重组事件数量揭示了菌株之间的重组。此外，通过系统发育分析和单倍型多样性评估两个物种同域分布的两个流行病灶中VL和CL之间的基因流，结果表明这两个物种之间没有基因交换。