Kazakhstan Scientific Center for Quarantine and Zoonotic Diseases, Almaty, Kazakhstan.
Emerg Infect Dis. 2010 May;16(5):789-96. doi: 10.3201/eid1605.091427.
To map the distribution of anthrax outbreaks and strain subtypes in Kazakhstan during 1937-2005, we combined geographic information system technology and genetic analysis by using archived cultures and data. Biochemical and genetic tests confirmed the identity of 93 archived cultures in the Kazakhstan National Culture Collection as Bacillus anthracis. Multilocus variable number tandem repeat analysis genotyping identified 12 genotypes. Cluster analysis comparing these genotypes with previously published genotypes indicated that most (n = 78) isolates belonged to the previously described A1.a genetic cluster, 6 isolates belonged to the A3.b cluster, and 2 belonged to the A4 cluster. Two genotypes in the collection appeared to represent novel genetic sublineages; 1 of these isolates was from Krygystan. Our data provide a description of the historical, geographic, and genetic diversity of B. anthracis in this Central Asian region.
为了绘制 1937-2005 年哈萨克斯坦炭疽疫情的分布和菌株亚型，我们结合地理信息系统技术和遗传分析，利用存档培养物和数据。生物化学和遗传测试证实，哈萨克斯坦国家文化收藏中的 93 个存档培养物为炭疽芽孢杆菌。多位点可变数串联重复分析基因分型确定了 12 种基因型。将这些基因型与之前发表的基因型进行聚类分析表明，大多数（n=78）分离株属于之前描述的 A1.a 遗传群，6 株属于 A3.b 群，2 株属于 A4 群。该收藏中的 2 种基因型似乎代表了新的遗传亚系；其中 1 株分离株来自吉尔吉斯斯坦。我们的数据提供了该中亚地区炭疽芽孢杆菌历史、地理和遗传多样性的描述。
