School of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China.
Bioresour Technol. 2010 Sep;101(18):7073-8. doi: 10.1016/j.biortech.2010.03.138. Epub 2010 Apr 20.
To improve the CO(2) fixation efficiency of non-photosynthetic microbial community (NPMC) isolated from sea water under aerobic conditions without hydrogen, the concentration of inorganic compounds as electron donors and their ratios were optimized using response surface methodology design (RSMD). These results indicated that Na(2)S, followed by Na(2)S(2)O(3) and NaNO(2) enhanced the CO(2) fixation by NPMC and the efficiency was increased about 100%, 200% and 200%, respectively. Some interaction between NaNO(2) and Na(2)S(2)O(3), as well as between Na(2)S(2)O(3) and Na(2)S was observed. Central composite RSMD experimentation predicted that the optimal concentration of these inorganic compounds and their ratios was 0.457% NaNO(2), 0.50% Na(2)S(2)O(3) and 1.25% Na(2)S. Under these conditions, the fixed CO(2) was 105.76 mg/L, which obviously exceeded the amount before optimization, as well as that obtained using hydrogen as the electron donor. This indicates that the NPMC using the established electron donors system can effectively fix CO(2) without light and hydrogen gas under aerobic condition.
为了提高从海水中分离出的非光合微生物群落（NPMC）在有氧条件下、无氢气存在时的 CO2 固定效率，采用响应面法设计（RSMD）优化了作为电子供体的无机化合物的浓度及其比例。结果表明，Na2S、Na2S2O3 和 NaNO2 依次提高了 NPMC 的 CO2 固定效率，分别提高了约 100%、200%和 200%。NaNO2 与 Na2S2O3 之间以及 Na2S2O3 与 Na2S 之间存在一些相互作用。中心复合 RSMD 实验预测，这些无机化合物的最佳浓度及其比例为 0.457% NaNO2、0.50% Na2S2O3 和 1.25% Na2S。在此条件下，固定的 CO2 为 105.76mg/L，明显超过了优化前的水平，也超过了使用氢气作为电子供体时的固定 CO2 量。这表明，使用所建立的电子供体系统的 NPMC 可以在有氧条件下无需光照和氢气有效地固定 CO2。
