Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, United States.
Semin Immunol. 2010 Jun;22(3):173-82. doi: 10.1016/j.smim.2010.03.002. Epub 2010 Apr 20.
Ex vivo generation and antigen loading of dendritic cells (DCs) from cancer patients helps to bypass the dysfunction of endogenous DCs. It also allows to control the process of DC maturation and to imprint in maturing DCs several functions essential for induction of effective forms of cancer immunity. Recent reports from several groups including ours demonstrate that distinct conditions of DC generation and maturation can prime DCs for preferential interaction with different (effector versus regulatory) subsets of immune cells. Moreover, differentially-generated DCs have been shown to imprint different effector mechanisms in CD4(+) and CD8(+) T cells (delivery of "signal three") and to induce their different homing properties (delivery of "signal four"). These developments allow for selective induction of tumor-specific T cells with desirable effector functions and tumor-relevant homing properties and to direct the desirable types of immune cells to tumors.
从癌症患者中体外生成和抗原负载树突状细胞 (DC) 有助于绕过内源性 DC 的功能障碍。它还允许控制 DC 成熟的过程，并在成熟的 DC 中植入诱导有效形式的癌症免疫所必需的几种功能。包括我们在内的几个研究小组的最新报告表明，不同的 DC 生成和成熟条件可以使 DC 优先与不同的（效应细胞与调节细胞）免疫细胞亚群相互作用。此外，已经证明，不同生成的 DC 可以在 CD4(+)和 CD8(+)T 细胞中诱导不同的效应机制（传递“信号三”），并诱导其不同的归巢特性（传递“信号四”）。这些发展使得可以选择性地诱导具有理想效应功能和与肿瘤相关归巢特性的肿瘤特异性 T 细胞，并将理想类型的免疫细胞引导至肿瘤。
