Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Wound Repair Regen. 2010 May-Jun;18(3):311-8. doi: 10.1111/j.1524-475X.2010.00586.x. Epub 2010 Apr 15.
The effect of burn blister fluid in neovascularization during burn wound healing is unknown. Burn blister fluid, containing a large amount of chemokines, is thought to play a role in the early stage of neovascularization. This process includes angiogenesis and vasculogenesis. Because of different healing time of burn wounds, we hypothesized that neovascularization in superficial partial thickness burn (SPTB) and deep partial thickness burn (DPTB) wounds were different. The neovasculogenic effects of two different burn blister fluids were also different. We found Day 7 DPTB wounds had a significant increase in blood vessels compared with SPTB wounds by immunohistochemistry. DPTB blister fluid significantly promoted neovascularization via increasing endothelial cell proliferation, and migration and differentiation of circulating angiogenic cells relative to SPTB blister fluids. In the animal study, DPTB blister fluids markedly promoted new blood vessel formation compared with those from SPTB blister fluids using in vivo Matrigel plug assay. These results suggest that DPTB wounds require more new vessel formation than SPTB. Furthermore, the measurement of angiogenic activities in burn blister fluids serves as a possible tool for assessing burn wound status.
烧伤水疱液在烧伤创面愈合过程中的促血管生成作用尚不清楚。烧伤水疱液中含有大量趋化因子，被认为在早期的血管生成过程中发挥作用。这个过程包括血管生成和血管发生。由于烧伤创面的愈合时间不同，我们假设浅二度烧伤（SPTB）和深二度烧伤（DPTB）创面的血管新生不同。两种不同的烧伤水疱液的促血管新生作用也不同。我们发现，免疫组化显示，第 7 天 DPTB 创面的血管数量明显多于 SPTB 创面。DPTB 水疱液通过增加内皮细胞增殖、循环血管生成细胞的迁移和分化，显著促进血管生成，与 SPTB 水疱液相比。在动物研究中，与 SPTB 水疱液相比，DPTB 水疱液在体内 Matrigel plugs 检测中明显促进了新血管的形成。这些结果表明，DPTB 创面比 SPTB 创面需要更多的新血管形成。此外，烧伤水疱液中血管生成活性的测量可以作为评估烧伤创面状态的一种可能工具。
