Chen Jian-Hua, Chen Yuan Zhong, Wu Yong
Fujian Institute of Hematology, Union Hospital, Fujian Medical University, Fuzhou 350001, Fujian Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2010 Apr;18(2):350-4.
This study was purposed to investigate the change of biological characteristics of HL-60 cells with high tumorigenicity transplanted through repeated passages into nude mice and to explore the tumorigenic mechanisms of the cell line. The human highly tumorigenic leukemia cell line HL-60 model in nude mice were established by serial passages in vivo and in vitro, and their biological features were compared. The trypan blue staining assay was used to detect the cell growth, the flow cytometry was used to analyze the cell cycle, the transmission electron microscopy and laser scanning confocal microscopy were used to observe the cell ultrastructures and cell fluorescence level respectively. The results indicated that the cell growth velocity was quickened, cell doubling time was shortened in high tumorigenic leukemia cell line HL-60; the cell count in S phase increased; the amount of mitochondria in HL-60 cells obviously decreased, furthermore the dilation of interspace, decrease of the number of ridges, vacuolation of mitochondria, significant reduction of fluorescence level in microfilament and enhancement of cell cloning efficacy were observed. It is concluded that the high tumorigenicity of HL-60 cells multiple-passaged in nude mice is associated with enhancement of proliferative ability, changes of number and structure of mitochondria in HL-60 cells, and alteration of microfilament in cytoskeleton.
本研究旨在探讨高致瘤性的HL-60细胞经多次传代接种于裸鼠后生物学特性的变化，并探讨该细胞系的致瘤机制。通过体内外连续传代建立人高致瘤性白血病细胞系HL-60裸鼠模型，并比较其生物学特性。采用台盼蓝染色法检测细胞生长情况，流式细胞术分析细胞周期，透射电子显微镜和激光扫描共聚焦显微镜分别观察细胞超微结构和细胞荧光水平。结果表明，高致瘤性白血病细胞系HL-60细胞生长速度加快，细胞倍增时间缩短；S期细胞数增加；HL-60细胞线粒体数量明显减少，线粒体间隙增宽、嵴数量减少、出现空泡化，微丝荧光水平显著降低，细胞克隆效率增强。结论是，在裸鼠体内多次传代的HL-60细胞的高致瘤性与增殖能力增强、HL-60细胞线粒体数量和结构改变以及细胞骨架中微丝改变有关。
