Stankovíc A, Front P, Barbara A, Mitrovíc D R
U-18 INSERM, Lariboisière Hospital, Paris, France.
Rheumatol Int. 1991;10(6):221-6. doi: 10.1007/BF02274882.
Monosodium urate monohydrate (MSUM) crystals derived from a tophus surgically removed from patients suffering from gout and MSUM prepared from a supersaturated solution of sodium urate were studied and compared with respect to their ability to: (1) stimulate chemiluminescence (CL) production by human polymorphonuclear (PMN) cells, (2) induce hemolysis of the human red blood cells and (3) induce inflammation when injected in the rat paw and knee joint. Human MSUM crystals were considerably more active in stimulating CL production by PMN cells and in inducing synovial inflammation. Both serum and papain pretreatment of human MSUM crystals caused inhibition of their enhancing effect on CL production by PMN cells. Papain pretreatment only reduced their phlogogenic activity. Uncoated and, to a much lesser extent, serum-coated human MSUM crystals induced secretion by mononuclear cells (MNC) of the factor(s) that considerably enhanced CL production by PMN cells. Both tophus-derived and synthetic crystals appeared to be weak hemolytic agents. Serum pretreatment of synthetic MSUM crystals reduced their hemolytic activity. These results suggest that surface coating, destroyed by papain treatment, was probably responsible for cell activation induced by human MSUM crystals.
对从痛风患者手术切除的痛风石中提取的尿酸单钠一水合物（MSUM）晶体以及由尿酸钠过饱和溶液制备的MSUM进行了研究，并比较了它们在以下方面的能力：（1）刺激人多形核（PMN）细胞产生化学发光（CL）；（2）诱导人红细胞溶血；（3）注射到大鼠爪和膝关节时诱导炎症。人MSUM晶体在刺激PMN细胞产生CL以及诱导滑膜炎方面的活性明显更高。血清和木瓜蛋白酶预处理人MSUM晶体均会抑制其对PMN细胞产生CL的增强作用。木瓜蛋白酶预处理仅降低了它们的致炎活性。未包被的以及程度小得多的血清包被的人MSUM晶体诱导单核细胞（MNC）分泌可显著增强PMN细胞产生CL的因子。痛风石来源的晶体和合成晶体似乎都是弱溶血剂。合成MSUM晶体的血清预处理降低了它们的溶血活性。这些结果表明，被木瓜蛋白酶处理破坏的表面包被可能是人MSUM晶体诱导细胞活化的原因。
