Dunnett M, Harris R C, Orme C E
Department of Comparative Physiology, Animal Health Trust, Newmarket, UK.
Scand J Clin Lab Invest. 1991 Apr;51(2):137-41. doi: 10.1080/00365519109091099.
A simple, robust and reproducible analytical method for the determination of phosphocreatine (PCr), creatine (Cr) and creatinine (Cn) in equine skeletal muscle is presented. The technique used isocratic reverse-phase ion-pairing high-performance liquid chromatography. Neutralized perchloric acid extracts of equine muscle biopsies were analysed and the values obtained were compared with determinations from an established enzymic procedure. Good resolution of all three metabolites was achieved within a retention time of less than 11 min. Linearity for each metabolite within the concentration range in the samples was demonstrated. Peak purity was specifically addressed. The abolition of each creatine in a pooled extract by enzymic incubation showed no underlying peaks. It was concluded that peaks were free of co-eluents which would otherwise lead to an overestimation of PCr, Cr and Cn concentrations.
本文介绍了一种用于测定马骨骼肌中磷酸肌酸（PCr）、肌酸（Cr）和肌酐（Cn）的简单、稳健且可重复的分析方法。所采用的技术是等度反相离子对高效液相色谱法。对马肌肉活检组织的中和高氯酸提取物进行分析，并将所得值与既定酶法测定结果进行比较。在不到11分钟的保留时间内实现了所有三种代谢物的良好分离。证明了样品浓度范围内每种代谢物的线性关系。特别关注了峰纯度。通过酶孵育消除合并提取物中的每种肌酸后未显示潜在峰。得出的结论是，峰中不存在共流出物，否则会导致PCr、Cr和Cn浓度的高估。
