Teng Hui, Mi Yani, Deng Hongting, He Yuanju, Wang Shunxin, Ai Chao, Cao Hui, Chen Lei
College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang 524088, China.
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Food Chem X. 2023 Jan 4;17:100559. doi: 10.1016/j.fochx.2023.100559. eCollection 2023 Mar 30.
Enzymatic acylation was employed to synthesize acylated anthocyanin, and a hybrid chemical model system was used for the formation of heterocyclic amines. And the inhibition effect and underline mechanism were investigated by analyzing the variations in important precursors and intermediates. Results confirmed that cyanidin-3-(6-cinnamoyl) -glycosidase (C3(6C)G) with a purity of 98.9% was obtained. HPLC identified seven types of heterocyclic amines (IQ, MeIQx, 4, 8-DimeiqX, Norharman, Harman, PhIP, and AαC) generated in the chemical model. (C3(6C)G) showed a good concentration-dependent manner for the inhibition effect on most HCAs except for MeIQx and PhIP. It also suppressed the glucose content, showed a dose-dependent manner in creatine/creatinine inhibition, and could scavenge formaldehyde, acetaldehyde, and phenylacetaldehyde. Two potential pathways might be involved: 1. by inhibiting the content of precursors (glucose and creatinine), competing with the formation of amino acids, to suppress HCAs generation; 2 through the removal of reactive carbonyl, reducing its reaction with creatinine.
采用酶促酰化反应合成酰化花青素，并使用混合化学模型系统生成杂环胺。通过分析重要前体和中间体的变化来研究抑制效果及潜在机制。结果证实获得了纯度为98.9%的矢车菊素-3-(6-肉桂酰基)-糖苷酶（C3(6C)G）。高效液相色谱法鉴定出化学模型中产生的七种杂环胺（IQ、MeIQx、4,8-二甲基IQx、去甲哈尔满、哈尔满、PhIP和AαC）。（C3(6C)G）对除MeIQx和PhIP之外的大多数杂环胺的抑制作用呈现出良好的浓度依赖性。它还能抑制葡萄糖含量，在肌酸/肌酐抑制方面呈剂量依赖性，并且能够清除甲醛、乙醛和苯乙醛。可能涉及两条潜在途径：1. 通过抑制前体（葡萄糖和肌酐）的含量，与氨基酸的形成竞争，以抑制杂环胺的生成；2. 通过去除活性羰基，减少其与肌酐的反应。
