Center for Infection and Immunity, Institute of Biophysics, Chinese Academy of Science, Beijing, China.
PLoS One. 2010 Apr 21;5(4):e10288. doi: 10.1371/journal.pone.0010288.
NKT cell is a population of unconventional T cells that mediate both innate and adaptive T cell responses. Since NKT cells are most abundant in the liver, much of NKT biology has been learnt from studies of NKT cells isolated from liver. This is a cumbersome procedure with variations in cell yield.
Based on recent evidence that NKT cells reside in liver vascular compartment, we developed a simple method to isolate NKT cells by perfusion with PBS-containing 10 mM of EDTA. The number and cell surface phenotype of liver NKT cells recovered by perfusion and by the traditional method were comparable. The yield of other lymphocytes was also comparable.
CONCLUSION/SIGNIFICANCE: Our data demonstrated that liver lymphocytes can be efficiently isolated by simple perfusion. These data provide a convenient method to isolate liver lymphocyte while preserving liver tissue for other analysis.
NKT 细胞是一种非常规 T 细胞群体，介导先天和适应性 T 细胞反应。由于 NKT 细胞在肝脏中最为丰富，因此，NKT 生物学的大部分内容都是从从肝脏中分离的 NKT 细胞研究中了解到的。这是一个繁琐的过程，细胞产量存在差异。
基于 NKT 细胞存在于肝脏血管腔室中的最新证据，我们开发了一种简单的方法，即用含有 10mM EDTA 的 PBS 进行灌流来分离 NKT 细胞。通过灌流和传统方法回收的肝脏 NKT 细胞的数量和细胞表面表型相当。其他淋巴细胞的产量也相当。
结论/意义：我们的数据表明，肝脏淋巴细胞可以通过简单的灌流有效地分离出来。这些数据提供了一种方便的方法来分离肝脏淋巴细胞，同时保留肝脏组织用于其他分析。
