Graduate Institute of Clinical Medicine, Taipei Medical University, Taipei 110, Taiwan, ROC.
Reproduction. 2010 Jul;140(1):105-12. doi: 10.1530/REP-09-0448. Epub 2010 Apr 27.
The applications of in vivo bioluminescent imaging (BLI) with a luciferase reporter gene occur widely across biomedical fields. Luciferase-transgenic mice are highly useful donors for tracking transplanted ovarian tissues. Realizing the full potential of this system may greatly benefit the study of the physiological behaviour and function of transplanted grafts, and the rapid and reliable evaluation of new transplantation protocols. The ovarian tissues of donor FVB/N-Tg(PolII-Luc)Ltc transgenic mice, with a luciferase transgene as the reporter, were transplanted into iso/allogeneic recipients. Rejection, ovarian function and BLI were quantitatively analysed in vivo over time. The BLI of the ovarian isografts revealed longer survival than that of allografts, even with cyclosporine A (CsA) treatment. The CD4(+)/CD8(+) ratios of peripheral T-cells were significantly reduced in allografts compared with those in isografts (P<0.0001) during rejection, whereas CD19(+) cell numbers were higher in allografts. The infiltration of CD4(+)/CD8(+) cells into the graft was unremarkable in isografts from day 1, but was strong in allografts from day 8 onwards. Hormone activity revealed complete oestrus cycles in the isografts but only the dioestrus stage in the allografts. These results demonstrate that BLI in vivo expedites the fast throughput and fate maps of ovarian grafts. The use of BLI to longitudinally monitor ovarian grafts for immunorejection demonstrated the short survival of allografts and the much longer survival of isografts. CsA treatment alone is ineffective against the acute rejection of ovarian allografts.
活体生物发光成像 (BLI) 应用广泛，可应用于生物医学领域。荧光素酶转基因小鼠是追踪移植卵巢组织的理想供体。充分利用这一系统可以极大地促进对移植移植物生理行为和功能的研究，以及对新移植方案的快速可靠评估。荧光素酶报告基因转基因 FVB/N-Tg(PolII-Luc)Ltc 小鼠的卵巢组织被移植到同种异体或异种受体中。通过活体体内分析，对排斥反应、卵巢功能和 BLI 进行了随时间的定量分析。BLI 分析显示，卵巢同种异体移植物的存活时间长于异种移植物，即使使用环孢素 A (CsA) 治疗也是如此。排斥反应期间，同种异体移植物的外周 T 细胞中 CD4(+)/CD8(+) 比值明显低于同种异体移植物（P<0.0001），而 CD19(+) 细胞数量较高。同种异体移植物中，CD4(+)/CD8(+) 细胞在第 1 天就大量浸润移植物，但在第 8 天开始的同种异体移植物中浸润情况明显增强。同种异体移植物中只出现间情期，而在同种异体移植物中则出现完全动情周期。这些结果表明，体内 BLI 加速了卵巢移植物的快速处理和命运图谱。使用 BLI 对卵巢移植物进行免疫排斥的纵向监测显示，同种异体移植物的存活时间短，而同种异体移植物的存活时间长。单独使用 CsA 治疗对卵巢同种异体移植物的急性排斥反应无效。
