The Center for Environmental Biotechnology, 676 Dabney Hall, The University of Tennessee, Knoxville, TN 37996, USA.
Sensors (Basel). 2011;11(1):180-206. doi: 10.3390/s110100180. Epub 2010 Dec 28.
In vivo bioluminescent imaging (BLI) is increasingly being utilized as a method for modern biological research. This process, which involves the noninvasive interrogation of living animals using light emitted from luciferase-expressing bioreporter cells, has been applied to study a wide range of biomolecular functions such as gene function, drug discovery and development, cellular trafficking, protein-protein interactions, and especially tumorigenesis, cancer treatment, and disease progression. This article will review the various bioreporter/biosensor integrations of BLI and discuss how BLI is being applied towards a new visual understanding of biological processes within the living organism.
体内生物发光成像（BLI）越来越多地被用作现代生物学研究的一种方法。该过程涉及使用来自表达荧光素酶的生物报告细胞发出的光对活体动物进行非侵入性询问，已被应用于研究广泛的生物分子功能，如基因功能、药物发现和开发、细胞运输、蛋白质-蛋白质相互作用，特别是肿瘤发生、癌症治疗和疾病进展。本文将综述 BLI 的各种生物报告器/生物传感器整合，并讨论 BLI 如何应用于对活体生物体内生物学过程的新的可视化理解。
