Sakata Naoaki, Obenaus Andre, Chan Nathaniel, Mace John, Chinnock Richard, Hathout Eba
Islet Transplant Laboratory; Department of Pediatrics; Loma Linda University School of Medicine; Loma Linda, CA USA.
Islets. 2009 Jul 1;1(1):26-33.
Embolic occlusion of the portal vein due to islet transplantation is one of the major reasons for reduced survival of transplanted islets. In this study, we examined the location of islets as well as the correlation between islet and portal vein size after intraportal islet transplantation and evaluated liver and islet pathology. RESULTS: The liver was divided into peripheral and central sites. Islet and liver apoptosis/necrosis were significantly higher at peripheral sites. In regions without liver apoptosis or necrosis, portal vein diameter was significantly larger and embolic ratios were significantly lower. METHODS: BALB/c mice were intraportally transplanted with 800 islets and the liver was examined at postoperative day (POD) 0 (n = 7), POD 2 (n = 4) and POD 28 (n = 3). Liver specimens were stained for hematoxylin and eosin (necrosis), insulin and TUNEL (apoptosis). We evaluated distance from liver surface to islets, islet and portal vein diameter, embolic ratio (islet diameter/portal vein diameter), apoptosis/necrosis of islets and apoptosis/necrosis of the liver tissue surrounding the islet. CONCLUSION: Transplanted islets and liver tissue exhibited more injury at peripheral sites, in part, due to smaller diameters of portal venules that result in more frequent emboli following islet transplantation.
胰岛移植导致的门静脉栓塞是移植胰岛存活率降低的主要原因之一。在本研究中，我们检查了门静脉内胰岛移植后胰岛的位置以及胰岛与门静脉大小之间的相关性，并评估了肝脏和胰岛的病理情况。结果：肝脏被分为外周和中央部位。外周部位的胰岛和肝脏凋亡/坏死明显更高。在没有肝脏凋亡或坏死的区域，门静脉直径明显更大，栓塞率明显更低。方法：将800个胰岛经门静脉移植到BALB/c小鼠体内，并在术后第0天（n = 7）、第2天（n = 4）和第28天（n = 3）检查肝脏。肝脏标本用苏木精和伊红染色（用于坏死）、胰岛素和TUNEL染色（用于凋亡）。我们评估了从肝脏表面到胰岛的距离、胰岛和门静脉直径、栓塞率（胰岛直径/门静脉直径）、胰岛的凋亡/坏死以及胰岛周围肝组织的凋亡/坏死。结论：移植的胰岛和肝组织在外周部位表现出更多损伤，部分原因是门静脉小分支直径较小，导致胰岛移植后栓塞更频繁。
