Islet Transplant Laboratory, Department of Pediatrics, Loma Linda University School of Medicine, Loma Linda, CA, USA.
Islets. 2009 Jul-Aug;1(1):26-33. doi: 10.4161/isl.1.1.8563.
Embolic occlusion of the portal vein due to islet transplantation is one of the major reasons for reduced survival of transplanted islets. In this study, we examined the location of islets as well as the correlation between islet and portal vein size after intraportal islet transplantation, and evaluated liver and islet pathology.
BALB/c mice were intraportally transplanted with 800 islets and the liver was examined at postoperative day (POD) 0 (n=7), POD 2 (n=4) and POD 28 (n=3). Liver specimens were stained for hematoxylin and eosin (necrosis), insulin, and TUNEL (apoptosis). We evaluated distance from liver surface to islets, islet and portal vein diameter, embolic ratio (islet diameter/portal vein diameter), apoptosis/necrosis of islets and apoptosis/necrosis of the liver tissue surrounding the islet.
The liver was divided into peripheral and central sites. Islet and liver apoptosis/necrosis were significantly higher at peripheral sites. In regions without liver apoptosis or necrosis, portal vein diameter was significantly larger and embolic ratios were significantly lower.
Transplanted islets and liver tissue exhibited more injury at peripheral sites, in part, due to smaller diameters of portal venules that result in more frequent emboli following islet transplantation.
胰岛移植后门静脉栓塞是移植胰岛存活率降低的主要原因之一。在本研究中，我们研究了门静脉内胰岛移植后胰岛的位置以及胰岛和门静脉大小之间的相关性，并评估了肝和胰岛的病理情况。
BALB/c 小鼠门静脉内移植 800 个胰岛，术后第 0 天（n=7）、第 2 天（n=4）和第 28 天（n=3）检查肝脏。肝标本行苏木精和伊红（坏死）、胰岛素和 TUNEL（凋亡）染色。我们评估了距肝表面的胰岛距离、胰岛和门静脉直径、栓塞比（胰岛直径/门静脉直径）、胰岛的凋亡/坏死以及胰岛周围肝组织的凋亡/坏死。
肝脏分为周边和中央部位。周边部位的胰岛和肝组织凋亡/坏死明显更高。在没有肝组织凋亡或坏死的区域，门静脉直径明显更大，栓塞比明显更低。
移植的胰岛和肝组织在周边部位表现出更多的损伤，部分原因是门静脉小静脉的直径较小，导致胰岛移植后更频繁地发生栓塞。
