Hathout Eba, Sowers Lawrence, Wang Rong, Tan Annie, Mace John, Peverini Ricardo, Chinnock Richard, Obenaus Andre
Islet Transplant Laboratory, Department of Pediatrics, Loma Linda University School of Medicine, CA, USA.
Transpl Int. 2007 Dec;20(12):1059-65. doi: 10.1111/j.1432-2277.2007.00550.x. Epub 2007 Sep 10.
To evaluate changes in neovascularization of transplanted islets in vivo, dynamic contrast (gadolinium) enhanced magnetic resonance imaging (MRI) was used. Both iron (Feridex)-labeled and unlabeled syngeneic murine subcapsular islet grafts were studied. Differences in dynamic contrast enhancement of islet grafts were quantified after gadolinium injection at post-transplant days 3 and 14. Normalized contrast concentrations at day 14 in transplanted islets were increased relative with that on day 3. Time to peak contrast enhancement was faster by 12 min at day 14 compared to day 3 islets (while kidney and muscle peak times remained the same). Areas under the curve for contrast concentration versus time plots were larger in 14-day relative to 3-day islet grafts. In conclusion, noninvasive assessment of neovascularization is achievable. In vivo dynamic contrast-enhanced MRI can be used to detect and quantify changes in vascularization following islet transplantation. This technique may be useful in developing pro-angiogenic strategies to improve the transplantation outcome in experimental and clinical settings.
为了评估体内移植胰岛新生血管形成的变化，采用了动态对比（钆）增强磁共振成像（MRI）。研究了铁（Feridex）标记和未标记的同基因小鼠被膜下胰岛移植。在移植后第3天和第14天注射钆后，对胰岛移植的动态对比增强差异进行了量化。移植胰岛在第14天的标准化对比剂浓度相对于第3天有所增加。与第3天的胰岛相比，第14天的胰岛对比剂增强峰值时间快了12分钟（而肾脏和肌肉的峰值时间保持不变）。与第3天的胰岛移植相比，第14天胰岛移植的对比剂浓度与时间曲线下面积更大。总之，无创评估新生血管形成是可行的。体内动态对比增强MRI可用于检测和量化胰岛移植后血管形成的变化。该技术可能有助于制定促血管生成策略，以改善实验和临床环境中的移植结果。
