Laboratório de Entomologia Médica, Instituto de Pesquisa René Rachou-Fiocruz, Belo Horizonte, MG, Brasil.
Mem Inst Oswaldo Cruz. 2010 Mar;105(2):174-8. doi: 10.1590/s0074-02762010000200011.
In this study, anticoagulant activity was detected in salivary gland homogenates (SGHs) of Thyrsopelma guianense (Diptera: Simuliidae). The SGH yielded 1.07 microg +/- 0.03 (n = 15) of total soluble protein per pair of glands. In addition, following SDS-PAGE (12.5% gel) and silver nitrate staining, 12 polypeptides with molecular weights ranging from 14-69 kDa were detected in all physiological ages analyzed (12 h, 24 h, 48 h and 72 h following emergence). Coagulation bioassays showed that the SGHs had activities that interacted at all levels of coagulation (the intrinsic, extrinsic and common pathways), by extending the plasma recalcification time, prothrombin time, thrombin time. This is the first report on the activity of salivary gland proteins from the main vector of onchocerciasis in Brazil. We also suggest detailed studies on the morphology and function of the salivary glands in order to understand the role of these proteins in host/vector interactions.
在这项研究中，我们检测到了 Thyrsopelma guianense（双翅目：Simuliidae）唾液腺匀浆（SGHs）中的抗凝活性。每个腺体产生 1.07 微克 +/- 0.03（n = 15）的总可溶性蛋白。此外，在 SDS-PAGE（12.5%凝胶）和硝酸银染色后，在所有分析的生理年龄（出芽后 12 小时、24 小时、48 小时和 72 小时）中均检测到 12 种分子量在 14-69 kDa 之间的多肽。凝血生物测定表明，SGHs 具有在所有凝血级联（内在、外在和共同途径）中相互作用的活性，通过延长血浆再钙化时间、凝血酶原时间和凝血酶时间。这是巴西主要盘尾丝虫病载体唾液腺蛋白活性的首次报道。我们还建议对唾液腺的形态和功能进行详细研究，以了解这些蛋白质在宿主/载体相互作用中的作用。
