来自美洲蚋（双翅目：蚋科）唾液腺的新型抗凝剂可抑制凝血因子V的活性。

Novel anticoagulant from salivary glands of Simulium vittatum (Diptera: Simuliidae) inhibits activity of coagulation factor V.

作者信息

Abebe M, Ribeiro J M, Cupp M S, Cupp E W

机构信息

Department of Entomology, University of Arizona, Tucson 85721, USA.

出版信息

J Med Entomol. 1996 Jan;33(1):173-6. doi: 10.1093/jmedent/33.1.173.

DOI:10.1093/jmedent/33.1.173

PMID:8906925

Abstract

Anticoagulant activity was detected in fractions of a reversed phase-high-performance liquid chromatography (RP-HPLC) of salivary gland lysate from Simulium vittatum Zetterstedt. Using a plasma recalcification time assay, these fractions did not inhibit factor Xa or thrombin. HPLC-purified fractions showing the anticoagulant property were pooled and examined using the activated partial thromboplastin time test conducted on normal plasma and plasmas deficient in factors V, VIII, IX, XI, and XII. The anticoagulant prolonged the clotting time of all the plasmas, except plasma deficient in factor V. The detection of antifactor V activity, together with other anticoagulants reported from Simulium spp. indicates a feeding strategy that targets enzymes in the terminal portion of the coagulation cascade.

摘要

在对美洲黑蝇（Simulium vittatum Zetterstedt）唾液腺裂解物进行反相高效液相色谱（RP-HPLC）分析时，在其组分中检测到了抗凝活性。使用血浆复钙时间测定法，这些组分并未抑制因子Xa或凝血酶。将显示出抗凝特性的HPLC纯化组分合并，并使用针对正常血浆以及缺乏因子V、VIII、IX、XI和XII的血浆进行的活化部分凝血活酶时间试验进行检测。该抗凝剂延长了所有血浆的凝血时间，但缺乏因子V的血浆除外。抗因子V活性的检测，以及从蚋属（Simulium spp.）报道的其他抗凝剂，表明了一种针对凝血级联末端部分酶的摄食策略。