NorChip AS, Klokkarstua, Norway.
Int J Oncol. 2010 Jun;36(6):1533-9. doi: 10.3892/ijo_00000640.
Detection of E6/E7 mRNA expression using the real-time nucleic acid sequence-based amplification assay (NASBA) PreTect HPV-Proofer was compared with results of human papillomavirus (HPV) DNA detection in 98 paraffin-embedded samples from patients with cervical adenocarcinoma. HR-HPV DNA was detected in 61 (62%), while HR-HPV E6/E7 mRNA was detected in 63 (64%) of the samples. Correlation between results from DNA analyses and the E6/E7 mRNA assay showed consistent results in 87% of samples (47 of 54). The results from these two methods in detecting presence of HPV infection of any type agreed in 77%. Overall agreement between the methods was seen in 82 of the 98 cases (84%). When evaluating change in sensitivity for detection of HPV positives by adding more HPV types to the HPV DNA assay, maximum sensitivity was reached by targeting four HPV types. The coverage of HPV DNA presence was 76.9%, while the E6/E7 mRNA assay achieved a maximum coverage of 80.8% using only three HPV types. Thus, E6/E7 oncogene expression analysis may provide a more objective test for assessment of neoplastic glandular cells. Further studies may reveal whether the clinical performance of the E6/E7 mRNA assay will be of prognostic value in management of cervical adenocarcinoma.
使用实时核酸序列扩增检测（NASBA）PreTect HPV-Proofer 检测 E6/E7 mRNA 表达，并与 98 例宫颈腺癌石蜡包埋样本中 HPV DNA 检测结果进行比较。61 例（62%）中检测到高危型 HPV DNA，63 例（64%）中检测到 HR-HPV E6/E7 mRNA。DNA 分析和 E6/E7 mRNA 检测结果之间的相关性显示，87%的样本（54 例中的 47 例）结果一致。两种方法在检测任何类型 HPV 感染的存在方面的总体一致性为 77%。在 98 例中，82 例（84%）方法一致。当通过将更多 HPV 类型添加到 HPV DNA 检测中以增加 HPV 阳性检测的敏感性时，针对四种 HPV 类型可达到最大敏感性。HPV DNA 存在的覆盖率为 76.9%，而仅使用三种 HPV 类型，E6/E7 mRNA 检测可实现最大覆盖率 80.8%。因此，E6/E7 致癌基因表达分析可能为评估肿瘤性腺体细胞提供更客观的检测方法。进一步的研究可能会揭示 E6/E7 mRNA 检测的临床性能是否对宫颈腺癌的管理具有预后价值。
