Meng Xianhua, Zhao Yinghui, Wang Jinyun, Gao Zheng, Geng Qingxia, Liu Xiaoxia
Department of Gynaecology and Obstetrics, Laiwu City People's Hospital, Laiwu, Shandong 271199, P.R. China.
Department of Gynaecology and Obstetrics, Laiwu Maternal and Child Care Hospital, Laiwu, Shandong 271100, P.R. China.
Exp Ther Med. 2017 Oct;14(4):2789-2794. doi: 10.3892/etm.2017.4887. Epub 2017 Aug 4.
The present study aimed to examine the role and underlying mechanism of miRNA-758 (miR-758) expression in cancer tissues, blood and cervical exfoliated cells from patients with cervical cancer. A total of 49 patients with cervical cancer and 26 healthy people for cervical cancer screening were included in the present study. The patients with cervical cancer were treated with resection, and the tumor and adjacent tissues, blood and cervical exfoliated cells were collected. The expression levels of miR-758 and matrix extracellular phosphoglycoprotein (MEPE) mRNA in each sample were detected by reverse transcription-quantitative polymerase chain reaction. In addition, western blot analysis was used to detect the MEPE protein in tumor tissues, while ELISA was applied to detect the MEPE protein expression in the blood and cervical exfoliated cells. Compared with the normal control, MEPE mRNA expression was upregulated in cervical cancer tissues, blood and cervical exfoliated cells. At the protein level, MEPE was also upregulated significantly in patients with cervical cancer. miR-758 expression was decreased significantly in cervical cancer tissues, blood and cervical exfoliated cells (P<0.05), which was opposite to the trend observed for MEPE mRNA expression. Furthermore, MEPE expression was increased in the tumor tissue, blood and cervical exfoliated cells of cervical cancer patients, which was associated to the downregulated miR-758. Therefore, miR-758 may regulate the infiltration and invasion of cervical cancer by targeting MEPE.
本研究旨在探讨微小RNA-758（miR-758）在宫颈癌患者癌组织、血液及宫颈脱落细胞中的作用及潜在机制。本研究共纳入49例宫颈癌患者及26例用于宫颈癌筛查的健康人。对宫颈癌患者进行手术切除治疗，并收集肿瘤组织及相邻组织、血液和宫颈脱落细胞。采用逆转录-定量聚合酶链反应检测各样本中miR-758和基质细胞外磷酸糖蛋白（MEPE）mRNA的表达水平。此外，采用蛋白质印迹法检测肿瘤组织中的MEPE蛋白，采用酶联免疫吸附测定法检测血液和宫颈脱落细胞中的MEPE蛋白表达。与正常对照相比，宫颈癌组织、血液和宫颈脱落细胞中MEPE mRNA表达上调。在蛋白质水平上，宫颈癌患者的MEPE也显著上调。miR-758在宫颈癌组织、血液和宫颈脱落细胞中的表达显著降低（P<0.05），这与MEPE mRNA表达的趋势相反。此外，宫颈癌患者肿瘤组织、血液和宫颈脱落细胞中的MEPE表达增加，这与miR-758下调有关。因此，miR-758可能通过靶向MEPE来调节宫颈癌的浸润和侵袭。
