Dipartimento di Chimica, Università di Perugia and Centro di Eccellenza sui Materiali Innovativi Nanostrutturati, Via Elce di Sotto 8, 06123 Perugia, Italy.
Langmuir. 2010 Jun 15;26(12):10129-34. doi: 10.1021/la100249t.
The luminescence behavior of CdS nanocrystals in aqueous solution and in the presence of proteins has been deeply investigated. CdS nanocrystals have been prepared in water by thermal decomposition of a single organometallic precursor assisted by thioglycerol, which acts as capping agent. Different experimental conditions have been explored to gain insights into the parameters affecting the nanocrystal growth. The CdS samples were characterized in terms of absorption and emission spectra, luminescence quantum yields, and decay times. These data together with size distribution analysis gave information on the growth mechanism and on the nature of the trap states formed in different experimental conditions. The emission properties of the nanocrystals in the presence of bovine serum albumin (BSA) have been examined to test how the electrostatic bioconjugation can influence the optical properties of the nanocrystals. The spectral changes observed upon addition of BSA indicated a direct interaction of the protein with the nanocrystal surface able to recover (at least partially) the defects formed during the crystal growth, resulting in improved emission properties.
我们深入研究了 CdS 纳米晶在水溶液中和存在蛋白质时的发光行为。CdS 纳米晶是通过热分解单一有机金属前体在巯基甘油的辅助下在水中制备的，巯基甘油作为封端剂。我们探索了不同的实验条件，以深入了解影响纳米晶生长的参数。我们对 CdS 样品进行了吸收和发射光谱、荧光量子产率和衰减时间的表征。这些数据以及尺寸分布分析提供了关于生长机制和在不同实验条件下形成的陷光态性质的信息。我们研究了纳米晶在牛血清白蛋白（BSA）存在下的发射特性，以检验静电生物共轭如何影响纳米晶的光学性质。加入 BSA 后观察到的光谱变化表明，蛋白质与纳米晶表面直接相互作用，能够（至少部分）恢复晶体生长过程中形成的缺陷，从而改善发射性能。
