Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais, Av. Antônio Carlos, 6627, School of Engineering, 31270-901, Belo Horizonte, MG, Brazil.
Colloids Surf B Biointerfaces. 2011 Jun 1;84(2):360-8. doi: 10.1016/j.colsurfb.2011.01.027. Epub 2011 Feb 25.
In the present work, it is reported for the first time the bioconjugation of CdS quantum dots (QDs) directly with bovine serum albumin (BSA) using a one-step procedure via aqueous route at room temperature by methods of colloidal chemistry. Essentially, the bioconjugates were developed based on BSA as capping ligand for the nucleation and stabilization of CdS nanoparticles using cadmium perchlorate and thioacetamide as precursors. UV-visible spectroscopy was used to characterize the kinetics and the relative stability of CdS nanoparticles. The CdS nanocrystals were produced with the calculated average particle size below 4.0 nm, indicating they were in the so-called "quantum-size confinement range". The results have clearly indicated that BSA was effective on nucleating and stabilizing the colloidal CdS quantum dots.
在本工作中，首次通过室温下的水相一步法，通过胶体化学方法，将 CdS 量子点（QDs）直接与牛血清白蛋白（BSA）偶联。本质上，基于 BSA 作为 CdS 纳米粒子成核和稳定的配体，以过氯酸镉和硫代乙酰胺为前体制备了生物缀合物。使用紫外-可见光谱来表征 CdS 纳米粒子的动力学和相对稳定性。CdS 纳米晶的平均粒径计算值低于 4.0nm，表明其处于所谓的“量子尺寸限制范围”内。结果清楚地表明，BSA 对胶体 CdS 量子点的成核和稳定是有效的。
