灵芝中一种新的水溶性中性多糖 GLP-F1-1 的纯化和结构表征。
Purification and structural characterization of a new water-soluble neutral polysaccharide GLP-F1-1 from Ganoderma lucidum.
机构信息
College of Light Industry and Food Sciences, South China University of Technology, Guangdong Province, Guangzhou 510640, China.
出版信息
Int J Biol Macromol. 2011 Jan 1;48(1):165-9. doi: 10.1016/j.ijbiomac.2010.10.015. Epub 2010 Oct 31.
A water-soluble neutral polysaccharide (GLP-F1-1) was isolated from the fruiting bodies of Ganoderma lucidum by DEAE Sepharose Fast Flow and Sephacryl S-500 High Resolution Chromatography. The neutral polysaccharide had an average molecular weight (Mw) of approximately 2.5×10(6) kDa. GC analysis showed that this polysaccharide was mainly composed of glucose and galactose in the molar ratio of 34:1. 1H and 13C NMR spectroscopy in combination with GC-MS technique indicated that the new polysaccharide had a backbone chain of 1,4-disubstituted-β-glucoseopyranose and 1,4,6-trisubstituted-β-glucoseopyranosyl, while the branched chains were mainly composed of 1,6-disubstituted-β-glucopyranosyl and 1,4-disubstituted-β-galactoseopyranosyl residues.
从灵芝子实体中分离到一种水溶性中性多糖(GLP-F1-1)，采用 DEAE Sepharose Fast Flow 和 Sephacryl S-500 High Resolution Chromatography 进行分离。该中性多糖的重均分子量（Mw）约为 2.5×10(6) kDa。GC 分析表明，该多糖主要由葡萄糖和半乳糖组成，摩尔比为 34:1。1H 和 13C NMR 光谱结合 GC-MS 技术表明，新多糖的主链由 1,4-二取代-β-吡喃葡萄糖和 1,4,6-三取代-β-吡喃葡萄糖组成，而支链主要由 1,6-二取代-β-吡喃葡萄糖和 1,4-二取代-β-吡喃半乳糖组成。
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