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[骨碎补根茎中黄酮类化合物微波提取条件优化实验]

[Condition optimization experiment of microwave extaction of flavonoids in rhizome of Drynaria fortunei].

作者信息

Yang Bin, Hu Fu-chao, Chen Gong-xi, Jiang Dao-song

机构信息

Jishou University/Key Laboratory of Plant Resources Conservation and Utilization of Hunan Province, Jishou 416000, China.

出版信息

Zhong Yao Cai. 2009 Dec;32(12):1907-10.

Abstract

The experiment extracted flavonoids in rhizome of Drynaria fortunei by microwave extraction, and determined the extraction rate through colorimetry. Through the single factor experiment and orthogonal method, the optimum extraction conditions were as follows: ethanol concentration was 40%, solid-liquid ratio was 1:20 (g/mL), microwave power was 325 W, extraction time was 40 s. Under these conditions, the extraction rate reached 1.73%. In all condtions, microwave power has the most significant effect on extraction rate. Microwave extraction has obvious advantages in comparison with traditional sovent refluxing method.

摘要

本实验采用微波萃取法从骨碎补根茎中提取黄酮类化合物,并通过比色法测定提取率。通过单因素实验和正交试验法,得出最佳提取条件如下:乙醇浓度为40%,固液比为1:20(g/mL),微波功率为325W,提取时间为40s。在此条件下,提取率达到1.73%。在所有条件中,微波功率对提取率的影响最为显著。与传统的溶剂回流法相比,微波萃取具有明显优势。

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