Patel B, Gove C D, Hutchinson W L, Mowbray J
Department of Biochemistry, University College London, U.K.
Biochim Biophys Acta. 1991 May 24;1074(1):178-81. doi: 10.1016/0304-4165(91)90058-o.
Perfusion with [8-14C]adenosine demonstrated the likely existence in rat liver of oligophosphoglyceroyl-ATP (OPG-ATP). Purification followed by assay with a new specific 3' phosphodiesterase confirmed this. The quantities present were 5-10-fold those found previously and comparable to total soluble nucleotides. OPG-ATP was also purified from the mitochondrial fraction, shown to co-distribute with succinate dehydrogenase and can be co-purified with an enzyme confined to intermembrane space.
用[8-14C]腺苷灌注表明大鼠肝脏中可能存在寡磷酸甘油酰-ATP（OPG-ATP）。通过用一种新的特异性3'磷酸二酯酶进行测定的纯化方法证实了这一点。其存在量是先前发现量的5至10倍，与总可溶性核苷酸相当。OPG-ATP也从线粒体部分中纯化出来，显示与琥珀酸脱氢酶共同分布，并且可以与一种局限于膜间隙的酶一起共纯化。
