Hutchinson W L, Ratcliffe P J, Mowbray J
Biochem J. 1986 Dec 1;240(2):597-9. doi: 10.1042/bj2400597.
The inability to account for large systematic variations in total purine nucleotide content of perfused rat hearts led to the demonstration that the soluble adenine nucleotides are in rapid equilibrium with a highly phosphorylated hetero-oligomeric derivative whose structure appears to be 3-phospho[glyceroyl-gamma-triphospho-5'-adenosine-3'-3-phosp ho]4glyceroyl- gamma-triphospho-5'-adenosine [Hutchinson, Morris & Mowbray (1986) Biochem. J. 234, 623-627]. Analogous techniques to those used with hearts for specifically labelling tissue purine nucleotides followed by extration and purification of nucleotides from the trichloroacetic acid-precipitable fraction show the existence of a corresponding rapid equilibrium between ATP and an oligomeric tetraphosphoadenosine derivative in perfused kidneys.
由于无法解释灌注大鼠心脏中总嘌呤核苷酸含量的巨大系统性变化，因此有研究表明，可溶性腺嘌呤核苷酸与一种高度磷酸化的杂寡聚衍生物处于快速平衡状态，其结构似乎是3-磷酸[甘油酰-γ-三磷酸-5'-腺苷-3'-3-磷酸]4甘油酰-γ-三磷酸-5'-腺苷[哈钦森、莫里斯和莫布雷（1986年）《生物化学杂志》234卷，623 - 627页]。用于心脏的类似技术，即先特异性标记组织嘌呤核苷酸，然后从三氯乙酸可沉淀部分提取和纯化核苷酸，结果表明在灌注肾脏中，ATP与一种寡聚四磷酸腺苷衍生物之间存在相应的快速平衡。
